Transferability and characterization of microsatellite markers in two Neotropical Ficus species

Detalhes bibliográficos
Autor(a) principal: Nazareno,Alison Gonçalves
Data de Publicação: 2009
Outros Autores: Pereira,Rodrigo Augusto Santinelo, Feres,Juliana Massimino, Mestriner,Moacyr Antônio, Alzate-Marin,Ana Lilia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300021
Resumo: Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87.
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spelling Transferability and characterization of microsatellite markers in two Neotropical Ficus speciesecological geneticsFicus citrifoliaFicus eximiaMoraceaeSSRsMicrosatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300021Genetics and Molecular Biology v.32 n.3 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000056info:eu-repo/semantics/openAccessNazareno,Alison GonçalvesPereira,Rodrigo Augusto SantineloFeres,Juliana MassiminoMestriner,Moacyr AntônioAlzate-Marin,Ana Liliaeng2009-08-10T00:00:00Zoai:scielo:S1415-47572009000300021Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-08-10T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Transferability and characterization of microsatellite markers in two Neotropical Ficus species
title Transferability and characterization of microsatellite markers in two Neotropical Ficus species
spellingShingle Transferability and characterization of microsatellite markers in two Neotropical Ficus species
Nazareno,Alison Gonçalves
ecological genetics
Ficus citrifolia
Ficus eximia
Moraceae
SSRs
title_short Transferability and characterization of microsatellite markers in two Neotropical Ficus species
title_full Transferability and characterization of microsatellite markers in two Neotropical Ficus species
title_fullStr Transferability and characterization of microsatellite markers in two Neotropical Ficus species
title_full_unstemmed Transferability and characterization of microsatellite markers in two Neotropical Ficus species
title_sort Transferability and characterization of microsatellite markers in two Neotropical Ficus species
author Nazareno,Alison Gonçalves
author_facet Nazareno,Alison Gonçalves
Pereira,Rodrigo Augusto Santinelo
Feres,Juliana Massimino
Mestriner,Moacyr Antônio
Alzate-Marin,Ana Lilia
author_role author
author2 Pereira,Rodrigo Augusto Santinelo
Feres,Juliana Massimino
Mestriner,Moacyr Antônio
Alzate-Marin,Ana Lilia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Nazareno,Alison Gonçalves
Pereira,Rodrigo Augusto Santinelo
Feres,Juliana Massimino
Mestriner,Moacyr Antônio
Alzate-Marin,Ana Lilia
dc.subject.por.fl_str_mv ecological genetics
Ficus citrifolia
Ficus eximia
Moraceae
SSRs
topic ecological genetics
Ficus citrifolia
Ficus eximia
Moraceae
SSRs
description Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572009005000056
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.32 n.3 2009
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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