SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100084 |
Resumo: | Abstract The white rhino is one of the great success stories of modern wildlife conservation, growing from as few as 50-100 animals in the 1880s, to approximately 20,000 white rhinoceros remaining today. However, illegal trade in conservational rhinoceros horns is adding constant pressure on remaining populations. Captive management of ex situ populations of endangered species using molecular methods can contribute to improving the management of the species. Here we compare for the first time the utility of 33 Single Nucleotide Polymorphisms (SNPs) and nine microsatellites (MS) in isolation and in combination for assigning parentage in captive White Rhinoceros. We found that a combined dataset of SNPs and microsatellites was most informative with the highest confidence level. This study thus provided us with a useful set of SNP and MS markers for parentage and relatedness testing. Further assessment of the utility of these markers over multiple (> three) generations and the incorporation of a larger variety of relationships among individuals (e.g. half-siblings or cousins) is strongly suggested. |
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Genetics and Molecular Biology |
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SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignmentSingle nucleotide polymorphismsmicrosatellite markersWhite RhinocerosCeratotherium simumAbstract The white rhino is one of the great success stories of modern wildlife conservation, growing from as few as 50-100 animals in the 1880s, to approximately 20,000 white rhinoceros remaining today. However, illegal trade in conservational rhinoceros horns is adding constant pressure on remaining populations. Captive management of ex situ populations of endangered species using molecular methods can contribute to improving the management of the species. Here we compare for the first time the utility of 33 Single Nucleotide Polymorphisms (SNPs) and nine microsatellites (MS) in isolation and in combination for assigning parentage in captive White Rhinoceros. We found that a combined dataset of SNPs and microsatellites was most informative with the highest confidence level. This study thus provided us with a useful set of SNP and MS markers for parentage and relatedness testing. Further assessment of the utility of these markers over multiple (> three) generations and the incorporation of a larger variety of relationships among individuals (e.g. half-siblings or cousins) is strongly suggested.Sociedade Brasileira de Genética2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100084Genetics and Molecular Biology v.40 n.1 2017reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2016-0058info:eu-repo/semantics/openAccessLabuschagne,ChristiaanDalton,Desiré L.Grobler,J. PaulKotzé,Antoinetteeng2017-04-25T00:00:00Zoai:scielo:S1415-47572017000100084Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2017-04-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment |
title |
SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment |
spellingShingle |
SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment Labuschagne,Christiaan Single nucleotide polymorphisms microsatellite markers White Rhinoceros Ceratotherium simum |
title_short |
SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment |
title_full |
SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment |
title_fullStr |
SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment |
title_full_unstemmed |
SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment |
title_sort |
SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment |
author |
Labuschagne,Christiaan |
author_facet |
Labuschagne,Christiaan Dalton,Desiré L. Grobler,J. Paul Kotzé,Antoinette |
author_role |
author |
author2 |
Dalton,Desiré L. Grobler,J. Paul Kotzé,Antoinette |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Labuschagne,Christiaan Dalton,Desiré L. Grobler,J. Paul Kotzé,Antoinette |
dc.subject.por.fl_str_mv |
Single nucleotide polymorphisms microsatellite markers White Rhinoceros Ceratotherium simum |
topic |
Single nucleotide polymorphisms microsatellite markers White Rhinoceros Ceratotherium simum |
description |
Abstract The white rhino is one of the great success stories of modern wildlife conservation, growing from as few as 50-100 animals in the 1880s, to approximately 20,000 white rhinoceros remaining today. However, illegal trade in conservational rhinoceros horns is adding constant pressure on remaining populations. Captive management of ex situ populations of endangered species using molecular methods can contribute to improving the management of the species. Here we compare for the first time the utility of 33 Single Nucleotide Polymorphisms (SNPs) and nine microsatellites (MS) in isolation and in combination for assigning parentage in captive White Rhinoceros. We found that a combined dataset of SNPs and microsatellites was most informative with the highest confidence level. This study thus provided us with a useful set of SNP and MS markers for parentage and relatedness testing. Further assessment of the utility of these markers over multiple (> three) generations and the incorporation of a larger variety of relationships among individuals (e.g. half-siblings or cousins) is strongly suggested. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100084 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100084 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2016-0058 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.40 n.1 2017 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122387526057984 |