Use of chromosome microdissection in fish molecular cytogenetics

Detalhes bibliográficos
Autor(a) principal: Henning,Frederico
Data de Publicação: 2008
Outros Autores: Trifonov,Vladimir, Almeida-Toledo,Lurdes Foresti de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200022
Resumo: Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these markers focusing on protocol modifications aiming to reduce the number of required chromosomes.
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spelling Use of chromosome microdissection in fish molecular cytogeneticsfluorescence in situ hybridizationchromosome paintingsex chromosomescross-FISHChromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these markers focusing on protocol modifications aiming to reduce the number of required chromosomes.Sociedade Brasileira de Genética2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200022Genetics and Molecular Biology v.31 n.1 suppl.0 2008reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572008000200022info:eu-repo/semantics/openAccessHenning,FredericoTrifonov,VladimirAlmeida-Toledo,Lurdes Foresti deeng2008-06-03T00:00:00Zoai:scielo:S1415-47572008000200022Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2008-06-03T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Use of chromosome microdissection in fish molecular cytogenetics
title Use of chromosome microdissection in fish molecular cytogenetics
spellingShingle Use of chromosome microdissection in fish molecular cytogenetics
Henning,Frederico
fluorescence in situ hybridization
chromosome painting
sex chromosomes
cross-FISH
title_short Use of chromosome microdissection in fish molecular cytogenetics
title_full Use of chromosome microdissection in fish molecular cytogenetics
title_fullStr Use of chromosome microdissection in fish molecular cytogenetics
title_full_unstemmed Use of chromosome microdissection in fish molecular cytogenetics
title_sort Use of chromosome microdissection in fish molecular cytogenetics
author Henning,Frederico
author_facet Henning,Frederico
Trifonov,Vladimir
Almeida-Toledo,Lurdes Foresti de
author_role author
author2 Trifonov,Vladimir
Almeida-Toledo,Lurdes Foresti de
author2_role author
author
dc.contributor.author.fl_str_mv Henning,Frederico
Trifonov,Vladimir
Almeida-Toledo,Lurdes Foresti de
dc.subject.por.fl_str_mv fluorescence in situ hybridization
chromosome painting
sex chromosomes
cross-FISH
topic fluorescence in situ hybridization
chromosome painting
sex chromosomes
cross-FISH
description Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these markers focusing on protocol modifications aiming to reduce the number of required chromosomes.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572008000200022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.31 n.1 suppl.0 2008
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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