Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)

Detalhes bibliográficos
Autor(a) principal: Kumar,Dinesh
Data de Publicação: 2006
Outros Autores: Gupta,Neelam, Ahlawat,S.P.S., Satyanarayana,R., Sunder,Shyam, Gupta,S.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200016
Resumo: The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect <FONT FACE=Symbol>a</font>-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the a-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These a-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection.
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spelling Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)alpha-lactalbumingenetic variabilitygoatsingle-strand conformation polymorphism (SSCP)The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect <FONT FACE=Symbol>a</font>-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the a-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These a-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection.Sociedade Brasileira de Genética2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200016Genetics and Molecular Biology v.29 n.2 2006reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572006000200016info:eu-repo/semantics/openAccessKumar,DineshGupta,NeelamAhlawat,S.P.S.Satyanarayana,R.Sunder,ShyamGupta,S.C.eng2006-06-12T00:00:00Zoai:scielo:S1415-47572006000200016Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2006-06-12T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
title Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
spellingShingle Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
Kumar,Dinesh
alpha-lactalbumin
genetic variability
goat
single-strand conformation polymorphism (SSCP)
title_short Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
title_full Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
title_fullStr Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
title_full_unstemmed Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
title_sort Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
author Kumar,Dinesh
author_facet Kumar,Dinesh
Gupta,Neelam
Ahlawat,S.P.S.
Satyanarayana,R.
Sunder,Shyam
Gupta,S.C.
author_role author
author2 Gupta,Neelam
Ahlawat,S.P.S.
Satyanarayana,R.
Sunder,Shyam
Gupta,S.C.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Kumar,Dinesh
Gupta,Neelam
Ahlawat,S.P.S.
Satyanarayana,R.
Sunder,Shyam
Gupta,S.C.
dc.subject.por.fl_str_mv alpha-lactalbumin
genetic variability
goat
single-strand conformation polymorphism (SSCP)
topic alpha-lactalbumin
genetic variability
goat
single-strand conformation polymorphism (SSCP)
description The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect <FONT FACE=Symbol>a</font>-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the a-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These a-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572006000200016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.29 n.2 2006
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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