Orf165 is associated with cytoplasmic male sterility in pepper

Detalhes bibliográficos
Autor(a) principal: Wen,Jin-fen
Data de Publicação: 2021
Outros Autores: Zhao,Kai, Lv,Jun-heng, Huo,Jin-long, Wang,Zi-ran, Wan,Hong-jian, Zhu,Hai-shan, Zhang,Zhu-qing, Shao,Gui-fang, Wang,Jiao, Zhang,Shui, Yang,Ting-yu, Zhang,Jing-rou, Zou,Xue-xiao, Deng,Ming-hua
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302
Resumo: Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper.
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spelling Orf165 is associated with cytoplasmic male sterility in pepperCapsicum annuum L.comparative transcriptomic analysestransgenic plantsmitochondrial targetingVIGSAbstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper.Sociedade Brasileira de Genética2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302Genetics and Molecular Biology v.44 n.3 2021reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2021-0030info:eu-repo/semantics/openAccessWen,Jin-fenZhao,KaiLv,Jun-hengHuo,Jin-longWang,Zi-ranWan,Hong-jianZhu,Hai-shanZhang,Zhu-qingShao,Gui-fangWang,JiaoZhang,ShuiYang,Ting-yuZhang,Jing-rouZou,Xue-xiaoDeng,Ming-huaeng2021-09-20T00:00:00Zoai:scielo:S1415-47572021000400302Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2021-09-20T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Orf165 is associated with cytoplasmic male sterility in pepper
title Orf165 is associated with cytoplasmic male sterility in pepper
spellingShingle Orf165 is associated with cytoplasmic male sterility in pepper
Wen,Jin-fen
Capsicum annuum L.
comparative transcriptomic analyses
transgenic plants
mitochondrial targeting
VIGS
title_short Orf165 is associated with cytoplasmic male sterility in pepper
title_full Orf165 is associated with cytoplasmic male sterility in pepper
title_fullStr Orf165 is associated with cytoplasmic male sterility in pepper
title_full_unstemmed Orf165 is associated with cytoplasmic male sterility in pepper
title_sort Orf165 is associated with cytoplasmic male sterility in pepper
author Wen,Jin-fen
author_facet Wen,Jin-fen
Zhao,Kai
Lv,Jun-heng
Huo,Jin-long
Wang,Zi-ran
Wan,Hong-jian
Zhu,Hai-shan
Zhang,Zhu-qing
Shao,Gui-fang
Wang,Jiao
Zhang,Shui
Yang,Ting-yu
Zhang,Jing-rou
Zou,Xue-xiao
Deng,Ming-hua
author_role author
author2 Zhao,Kai
Lv,Jun-heng
Huo,Jin-long
Wang,Zi-ran
Wan,Hong-jian
Zhu,Hai-shan
Zhang,Zhu-qing
Shao,Gui-fang
Wang,Jiao
Zhang,Shui
Yang,Ting-yu
Zhang,Jing-rou
Zou,Xue-xiao
Deng,Ming-hua
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wen,Jin-fen
Zhao,Kai
Lv,Jun-heng
Huo,Jin-long
Wang,Zi-ran
Wan,Hong-jian
Zhu,Hai-shan
Zhang,Zhu-qing
Shao,Gui-fang
Wang,Jiao
Zhang,Shui
Yang,Ting-yu
Zhang,Jing-rou
Zou,Xue-xiao
Deng,Ming-hua
dc.subject.por.fl_str_mv Capsicum annuum L.
comparative transcriptomic analyses
transgenic plants
mitochondrial targeting
VIGS
topic Capsicum annuum L.
comparative transcriptomic analyses
transgenic plants
mitochondrial targeting
VIGS
description Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2021-0030
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.44 n.3 2021
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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