Orf165 is associated with cytoplasmic male sterility in pepper
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302 |
Resumo: | Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper. |
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Genetics and Molecular Biology |
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Orf165 is associated with cytoplasmic male sterility in pepperCapsicum annuum L.comparative transcriptomic analysestransgenic plantsmitochondrial targetingVIGSAbstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper.Sociedade Brasileira de Genética2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302Genetics and Molecular Biology v.44 n.3 2021reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2021-0030info:eu-repo/semantics/openAccessWen,Jin-fenZhao,KaiLv,Jun-hengHuo,Jin-longWang,Zi-ranWan,Hong-jianZhu,Hai-shanZhang,Zhu-qingShao,Gui-fangWang,JiaoZhang,ShuiYang,Ting-yuZhang,Jing-rouZou,Xue-xiaoDeng,Ming-huaeng2021-09-20T00:00:00Zoai:scielo:S1415-47572021000400302Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2021-09-20T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Orf165 is associated with cytoplasmic male sterility in pepper |
title |
Orf165 is associated with cytoplasmic male sterility in pepper |
spellingShingle |
Orf165 is associated with cytoplasmic male sterility in pepper Wen,Jin-fen Capsicum annuum L. comparative transcriptomic analyses transgenic plants mitochondrial targeting VIGS |
title_short |
Orf165 is associated with cytoplasmic male sterility in pepper |
title_full |
Orf165 is associated with cytoplasmic male sterility in pepper |
title_fullStr |
Orf165 is associated with cytoplasmic male sterility in pepper |
title_full_unstemmed |
Orf165 is associated with cytoplasmic male sterility in pepper |
title_sort |
Orf165 is associated with cytoplasmic male sterility in pepper |
author |
Wen,Jin-fen |
author_facet |
Wen,Jin-fen Zhao,Kai Lv,Jun-heng Huo,Jin-long Wang,Zi-ran Wan,Hong-jian Zhu,Hai-shan Zhang,Zhu-qing Shao,Gui-fang Wang,Jiao Zhang,Shui Yang,Ting-yu Zhang,Jing-rou Zou,Xue-xiao Deng,Ming-hua |
author_role |
author |
author2 |
Zhao,Kai Lv,Jun-heng Huo,Jin-long Wang,Zi-ran Wan,Hong-jian Zhu,Hai-shan Zhang,Zhu-qing Shao,Gui-fang Wang,Jiao Zhang,Shui Yang,Ting-yu Zhang,Jing-rou Zou,Xue-xiao Deng,Ming-hua |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Wen,Jin-fen Zhao,Kai Lv,Jun-heng Huo,Jin-long Wang,Zi-ran Wan,Hong-jian Zhu,Hai-shan Zhang,Zhu-qing Shao,Gui-fang Wang,Jiao Zhang,Shui Yang,Ting-yu Zhang,Jing-rou Zou,Xue-xiao Deng,Ming-hua |
dc.subject.por.fl_str_mv |
Capsicum annuum L. comparative transcriptomic analyses transgenic plants mitochondrial targeting VIGS |
topic |
Capsicum annuum L. comparative transcriptomic analyses transgenic plants mitochondrial targeting VIGS |
description |
Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2021-0030 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.44 n.3 2021 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122390529179648 |