A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100030 |
Resumo: | Population size and phenotypic measurement are two key factors determining the detection power of quantitative trait loci (QTL) mapping. We evaluated how these two controllable factors quantitatively affect the detection of QTL and their localization using a large F2 murine mapping population and found that three main points emerged from this study. One finding was that the sensitivity of QTL detection significantly decreased as the population size decreased. The decrease in the percentage logarithm of the odd score (LOD score, which is a statistical measure of the likelihood of two loci being lied near each other on a chromosome) can be estimated using the formula 1 - n/N, where n is the smaller and N the larger population size. This empirical formula has several practical implications in QTL mapping. We also found that a population size of 300 seems to be a threshold for the detection of QTL and their localization, which challenges the small population sizes commonly-used in published studies, in excess of 60% of which cite population sizes <300. In addition, it seems that the precision of phenotypic measurement has a limited capacity to affect detection power, which means that quantitative traits that cannot be measured precisely can also be used in QTL mapping for the detection of major QTL. |
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Genetics and Molecular Biology |
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A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping populationQTL mappingmicedetection powerpopulation sizephenotypic measurementPopulation size and phenotypic measurement are two key factors determining the detection power of quantitative trait loci (QTL) mapping. We evaluated how these two controllable factors quantitatively affect the detection of QTL and their localization using a large F2 murine mapping population and found that three main points emerged from this study. One finding was that the sensitivity of QTL detection significantly decreased as the population size decreased. The decrease in the percentage logarithm of the odd score (LOD score, which is a statistical measure of the likelihood of two loci being lied near each other on a chromosome) can be estimated using the formula 1 - n/N, where n is the smaller and N the larger population size. This empirical formula has several practical implications in QTL mapping. We also found that a population size of 300 seems to be a threshold for the detection of QTL and their localization, which challenges the small population sizes commonly-used in published studies, in excess of 60% of which cite population sizes <300. In addition, it seems that the precision of phenotypic measurement has a limited capacity to affect detection power, which means that quantitative traits that cannot be measured precisely can also be used in QTL mapping for the detection of major QTL.Sociedade Brasileira de Genética2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100030Genetics and Molecular Biology v.29 n.1 2006reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572006000100030info:eu-repo/semantics/openAccessLi,XinminQuigg,Richard JZhou,JianXu,ShizhongMasinde,GodfredMohan,SubburamanBaylink,David J.eng2006-03-10T00:00:00Zoai:scielo:S1415-47572006000100030Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2006-03-10T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population |
title |
A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population |
spellingShingle |
A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population Li,Xinmin QTL mapping mice detection power population size phenotypic measurement |
title_short |
A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population |
title_full |
A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population |
title_fullStr |
A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population |
title_full_unstemmed |
A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population |
title_sort |
A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population |
author |
Li,Xinmin |
author_facet |
Li,Xinmin Quigg,Richard J Zhou,Jian Xu,Shizhong Masinde,Godfred Mohan,Subburaman Baylink,David J. |
author_role |
author |
author2 |
Quigg,Richard J Zhou,Jian Xu,Shizhong Masinde,Godfred Mohan,Subburaman Baylink,David J. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Li,Xinmin Quigg,Richard J Zhou,Jian Xu,Shizhong Masinde,Godfred Mohan,Subburaman Baylink,David J. |
dc.subject.por.fl_str_mv |
QTL mapping mice detection power population size phenotypic measurement |
topic |
QTL mapping mice detection power population size phenotypic measurement |
description |
Population size and phenotypic measurement are two key factors determining the detection power of quantitative trait loci (QTL) mapping. We evaluated how these two controllable factors quantitatively affect the detection of QTL and their localization using a large F2 murine mapping population and found that three main points emerged from this study. One finding was that the sensitivity of QTL detection significantly decreased as the population size decreased. The decrease in the percentage logarithm of the odd score (LOD score, which is a statistical measure of the likelihood of two loci being lied near each other on a chromosome) can be estimated using the formula 1 - n/N, where n is the smaller and N the larger population size. This empirical formula has several practical implications in QTL mapping. We also found that a population size of 300 seems to be a threshold for the detection of QTL and their localization, which challenges the small population sizes commonly-used in published studies, in excess of 60% of which cite population sizes <300. In addition, it seems that the precision of phenotypic measurement has a limited capacity to affect detection power, which means that quantitative traits that cannot be measured precisely can also be used in QTL mapping for the detection of major QTL. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100030 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100030 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572006000100030 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.29 n.1 2006 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122379885084672 |