Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata

Detalhes bibliográficos
Autor(a) principal: Jamsari,Amirul Firdaus Jamaluddin
Data de Publicação: 2011
Outros Autores: Min-Pau,Tan, Siti-Azizah,Mohd Nor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200029
Resumo: Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.
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spelling Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striataChanna striatapopulation geneticsmicrosatellitemultiplex PCRSeven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.Sociedade Brasileira de Genética2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200029Genetics and Molecular Biology v.34 n.2 2011reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572011005000007info:eu-repo/semantics/openAccessJamsari,Amirul Firdaus JamaluddinMin-Pau,TanSiti-Azizah,Mohd Noreng2011-06-02T00:00:00Zoai:scielo:S1415-47572011000200029Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2011-06-02T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
spellingShingle Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
Jamsari,Amirul Firdaus Jamaluddin
Channa striata
population genetics
microsatellite
multiplex PCR
title_short Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title_full Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title_fullStr Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title_full_unstemmed Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title_sort Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
author Jamsari,Amirul Firdaus Jamaluddin
author_facet Jamsari,Amirul Firdaus Jamaluddin
Min-Pau,Tan
Siti-Azizah,Mohd Nor
author_role author
author2 Min-Pau,Tan
Siti-Azizah,Mohd Nor
author2_role author
author
dc.contributor.author.fl_str_mv Jamsari,Amirul Firdaus Jamaluddin
Min-Pau,Tan
Siti-Azizah,Mohd Nor
dc.subject.por.fl_str_mv Channa striata
population genetics
microsatellite
multiplex PCR
topic Channa striata
population genetics
microsatellite
multiplex PCR
description Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200029
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572011005000007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.34 n.2 2011
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
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reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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