In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos

Detalhes bibliográficos
Autor(a) principal: Gabriel,Jane E.
Data de Publicação: 2000
Outros Autores: Alves,Helena J., Alvares,Lucia E., Schmidt,Gilberto S., Coutinho,Luiz L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100027
Resumo: In situ hybridization has provided insights into the molecular basis of skeletal myogenesis during embryonic development. In situ detection of different muscle-specific regulatory factors in whole embryos has been described. Spatial and temporal expression patterns of these factors differed among species. The expression pattern of MyoD in whole chicken embryos was studied via in situ hybridization using a probe obtained by the reverse transcription - polymerase chain reaction (RT-PCR) technique. In newly formed somites (embryos of stage 12), MyoD mRNA transcripts were detected along the anterior to posterior axis of somites immediately adjacent to the neural tube, whereas in mature somites (embryos of stage 24), MyoD transcripts were detected throughout the entire somite. These results indicate that MyoD expression is important for initiating and maintaining the avian myogenic system.
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spelling In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryosIn situ hybridization has provided insights into the molecular basis of skeletal myogenesis during embryonic development. In situ detection of different muscle-specific regulatory factors in whole embryos has been described. Spatial and temporal expression patterns of these factors differed among species. The expression pattern of MyoD in whole chicken embryos was studied via in situ hybridization using a probe obtained by the reverse transcription - polymerase chain reaction (RT-PCR) technique. In newly formed somites (embryos of stage 12), MyoD mRNA transcripts were detected along the anterior to posterior axis of somites immediately adjacent to the neural tube, whereas in mature somites (embryos of stage 24), MyoD transcripts were detected throughout the entire somite. These results indicate that MyoD expression is important for initiating and maintaining the avian myogenic system.Sociedade Brasileira de Genética2000-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100027Genetics and Molecular Biology v.23 n.1 2000reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572000000100027info:eu-repo/semantics/openAccessGabriel,Jane E.Alves,Helena J.Alvares,Lucia E.Schmidt,Gilberto S.Coutinho,Luiz L.eng2000-08-25T00:00:00Zoai:scielo:S1415-47572000000100027Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2000-08-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos
title In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos
spellingShingle In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos
Gabriel,Jane E.
title_short In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos
title_full In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos
title_fullStr In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos
title_full_unstemmed In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos
title_sort In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos
author Gabriel,Jane E.
author_facet Gabriel,Jane E.
Alves,Helena J.
Alvares,Lucia E.
Schmidt,Gilberto S.
Coutinho,Luiz L.
author_role author
author2 Alves,Helena J.
Alvares,Lucia E.
Schmidt,Gilberto S.
Coutinho,Luiz L.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gabriel,Jane E.
Alves,Helena J.
Alvares,Lucia E.
Schmidt,Gilberto S.
Coutinho,Luiz L.
description In situ hybridization has provided insights into the molecular basis of skeletal myogenesis during embryonic development. In situ detection of different muscle-specific regulatory factors in whole embryos has been described. Spatial and temporal expression patterns of these factors differed among species. The expression pattern of MyoD in whole chicken embryos was studied via in situ hybridization using a probe obtained by the reverse transcription - polymerase chain reaction (RT-PCR) technique. In newly formed somites (embryos of stage 12), MyoD mRNA transcripts were detected along the anterior to posterior axis of somites immediately adjacent to the neural tube, whereas in mature somites (embryos of stage 24), MyoD transcripts were detected throughout the entire somite. These results indicate that MyoD expression is important for initiating and maintaining the avian myogenic system.
publishDate 2000
dc.date.none.fl_str_mv 2000-03-01
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100027
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572000000100027
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.23 n.1 2000
reponame:Genetics and Molecular Biology
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