Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021 |
Resumo: | The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased miR-140-5p expression (p = 0.009). miR-140-5p expression was elevated in invasive ductal carcinomas (p = 0.002), whereas basal-like tumors had decreased expression of miR-140-5p compared to other tumors (p = 0.008). Lymph node-positive samples showed an approximately 13-fold increase in miR-140-5p expression compared to lymph node-negative tissue (p = 0.049). These findings suggest that miR-140-5p, but not miR-193b, could be an important determinant of IGFBP5 expression and clinical phenotype in breast cancer patients. Further studies are needed to clarify the expressional regulation of IGFBP5 by miR-140-5p. |
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Genetics and Molecular Biology |
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Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5breast cancerER alphaIGFBP5micro RNAmiR-140miR-193bThe functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased miR-140-5p expression (p = 0.009). miR-140-5p expression was elevated in invasive ductal carcinomas (p = 0.002), whereas basal-like tumors had decreased expression of miR-140-5p compared to other tumors (p = 0.008). Lymph node-positive samples showed an approximately 13-fold increase in miR-140-5p expression compared to lymph node-negative tissue (p = 0.049). These findings suggest that miR-140-5p, but not miR-193b, could be an important determinant of IGFBP5 expression and clinical phenotype in breast cancer patients. Further studies are needed to clarify the expressional regulation of IGFBP5 by miR-140-5p.Sociedade Brasileira de Genética2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021Genetics and Molecular Biology v.38 n.1 2015reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-475738120140167info:eu-repo/semantics/openAccessGüllü,GökçePeker,IremHaholu,AptullahEren,FatihKüçükodaci,ZaferGüleç,BülentBaloglu,HüseyinErzik,CanÖzer,AyseAkkiprik,Mustafaeng2015-04-22T00:00:00Zoai:scielo:S1415-47572015000100021Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2015-04-22T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5 |
title |
Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5 |
spellingShingle |
Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5 Güllü,Gökçe breast cancer ER alpha IGFBP5 micro RNA miR-140 miR-193b |
title_short |
Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5 |
title_full |
Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5 |
title_fullStr |
Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5 |
title_full_unstemmed |
Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5 |
title_sort |
Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5 |
author |
Güllü,Gökçe |
author_facet |
Güllü,Gökçe Peker,Irem Haholu,Aptullah Eren,Fatih Küçükodaci,Zafer Güleç,Bülent Baloglu,Hüseyin Erzik,Can Özer,Ayse Akkiprik,Mustafa |
author_role |
author |
author2 |
Peker,Irem Haholu,Aptullah Eren,Fatih Küçükodaci,Zafer Güleç,Bülent Baloglu,Hüseyin Erzik,Can Özer,Ayse Akkiprik,Mustafa |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Güllü,Gökçe Peker,Irem Haholu,Aptullah Eren,Fatih Küçükodaci,Zafer Güleç,Bülent Baloglu,Hüseyin Erzik,Can Özer,Ayse Akkiprik,Mustafa |
dc.subject.por.fl_str_mv |
breast cancer ER alpha IGFBP5 micro RNA miR-140 miR-193b |
topic |
breast cancer ER alpha IGFBP5 micro RNA miR-140 miR-193b |
description |
The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased miR-140-5p expression (p = 0.009). miR-140-5p expression was elevated in invasive ductal carcinomas (p = 0.002), whereas basal-like tumors had decreased expression of miR-140-5p compared to other tumors (p = 0.008). Lymph node-positive samples showed an approximately 13-fold increase in miR-140-5p expression compared to lymph node-negative tissue (p = 0.049). These findings suggest that miR-140-5p, but not miR-193b, could be an important determinant of IGFBP5 expression and clinical phenotype in breast cancer patients. Further studies are needed to clarify the expressional regulation of IGFBP5 by miR-140-5p. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-475738120140167 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.38 n.1 2015 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122386256232448 |