Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5

Detalhes bibliográficos
Autor(a) principal: Güllü,Gökçe
Data de Publicação: 2015
Outros Autores: Peker,Irem, Haholu,Aptullah, Eren,Fatih, Küçükodaci,Zafer, Güleç,Bülent, Baloglu,Hüseyin, Erzik,Can, Özer,Ayse, Akkiprik,Mustafa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021
Resumo: The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased miR-140-5p expression (p = 0.009). miR-140-5p expression was elevated in invasive ductal carcinomas (p = 0.002), whereas basal-like tumors had decreased expression of miR-140-5p compared to other tumors (p = 0.008). Lymph node-positive samples showed an approximately 13-fold increase in miR-140-5p expression compared to lymph node-negative tissue (p = 0.049). These findings suggest that miR-140-5p, but not miR-193b, could be an important determinant of IGFBP5 expression and clinical phenotype in breast cancer patients. Further studies are needed to clarify the expressional regulation of IGFBP5 by miR-140-5p.
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spelling Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5breast cancerER alphaIGFBP5micro RNAmiR-140miR-193bThe functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased miR-140-5p expression (p = 0.009). miR-140-5p expression was elevated in invasive ductal carcinomas (p = 0.002), whereas basal-like tumors had decreased expression of miR-140-5p compared to other tumors (p = 0.008). Lymph node-positive samples showed an approximately 13-fold increase in miR-140-5p expression compared to lymph node-negative tissue (p = 0.049). These findings suggest that miR-140-5p, but not miR-193b, could be an important determinant of IGFBP5 expression and clinical phenotype in breast cancer patients. Further studies are needed to clarify the expressional regulation of IGFBP5 by miR-140-5p.Sociedade Brasileira de Genética2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021Genetics and Molecular Biology v.38 n.1 2015reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-475738120140167info:eu-repo/semantics/openAccessGüllü,GökçePeker,IremHaholu,AptullahEren,FatihKüçükodaci,ZaferGüleç,BülentBaloglu,HüseyinErzik,CanÖzer,AyseAkkiprik,Mustafaeng2015-04-22T00:00:00Zoai:scielo:S1415-47572015000100021Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2015-04-22T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
title Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
spellingShingle Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
Güllü,Gökçe
breast cancer
ER alpha
IGFBP5
micro RNA
miR-140
miR-193b
title_short Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
title_full Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
title_fullStr Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
title_full_unstemmed Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
title_sort Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
author Güllü,Gökçe
author_facet Güllü,Gökçe
Peker,Irem
Haholu,Aptullah
Eren,Fatih
Küçükodaci,Zafer
Güleç,Bülent
Baloglu,Hüseyin
Erzik,Can
Özer,Ayse
Akkiprik,Mustafa
author_role author
author2 Peker,Irem
Haholu,Aptullah
Eren,Fatih
Küçükodaci,Zafer
Güleç,Bülent
Baloglu,Hüseyin
Erzik,Can
Özer,Ayse
Akkiprik,Mustafa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Güllü,Gökçe
Peker,Irem
Haholu,Aptullah
Eren,Fatih
Küçükodaci,Zafer
Güleç,Bülent
Baloglu,Hüseyin
Erzik,Can
Özer,Ayse
Akkiprik,Mustafa
dc.subject.por.fl_str_mv breast cancer
ER alpha
IGFBP5
micro RNA
miR-140
miR-193b
topic breast cancer
ER alpha
IGFBP5
micro RNA
miR-140
miR-193b
description The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased miR-140-5p expression (p = 0.009). miR-140-5p expression was elevated in invasive ductal carcinomas (p = 0.002), whereas basal-like tumors had decreased expression of miR-140-5p compared to other tumors (p = 0.008). Lymph node-positive samples showed an approximately 13-fold increase in miR-140-5p expression compared to lymph node-negative tissue (p = 0.049). These findings suggest that miR-140-5p, but not miR-193b, could be an important determinant of IGFBP5 expression and clinical phenotype in breast cancer patients. Further studies are needed to clarify the expressional regulation of IGFBP5 by miR-140-5p.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-475738120140167
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.38 n.1 2015
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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