Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women

Detalhes bibliográficos
Autor(a) principal: Oliveira,Jéssica de
Data de Publicação: 2017
Outros Autores: Tureck,Luciane Viater, Santos,Willian dos, Saliba,Louise Farah, Schenknecht,Caroline Schovanz, Scaraboto,Débora, Souza,Ricardo Lehtonen R., Furtado-Alle,Lupe
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408
Resumo: Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.
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spelling Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in womenBCHE geneobesitylipid metabolismpolymorphismsAbstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.Sociedade Brasileira de Genética2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408Genetics and Molecular Biology v.40 n.2 2017reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2016-0123info:eu-repo/semantics/openAccessOliveira,Jéssica deTureck,Luciane ViaterSantos,Willian dosSaliba,Louise FarahSchenknecht,Caroline SchovanzScaraboto,DéboraSouza,Ricardo Lehtonen R.Furtado-Alle,Lupeeng2017-06-28T00:00:00Zoai:scielo:S1415-47572017000300408Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2017-06-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
spellingShingle Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
Oliveira,Jéssica de
BCHE gene
obesity
lipid metabolism
polymorphisms
title_short Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title_full Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title_fullStr Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title_full_unstemmed Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title_sort Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
author Oliveira,Jéssica de
author_facet Oliveira,Jéssica de
Tureck,Luciane Viater
Santos,Willian dos
Saliba,Louise Farah
Schenknecht,Caroline Schovanz
Scaraboto,Débora
Souza,Ricardo Lehtonen R.
Furtado-Alle,Lupe
author_role author
author2 Tureck,Luciane Viater
Santos,Willian dos
Saliba,Louise Farah
Schenknecht,Caroline Schovanz
Scaraboto,Débora
Souza,Ricardo Lehtonen R.
Furtado-Alle,Lupe
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Jéssica de
Tureck,Luciane Viater
Santos,Willian dos
Saliba,Louise Farah
Schenknecht,Caroline Schovanz
Scaraboto,Débora
Souza,Ricardo Lehtonen R.
Furtado-Alle,Lupe
dc.subject.por.fl_str_mv BCHE gene
obesity
lipid metabolism
polymorphisms
topic BCHE gene
obesity
lipid metabolism
polymorphisms
description Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2016-0123
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.40 n.2 2017
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
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reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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