PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei

Detalhes bibliográficos
Autor(a) principal: Freitas,Patrícia Domingues de
Data de Publicação: 2002
Outros Autores: Galetti Junior,Pedro Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012
Resumo: The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain could account for the reduced adaptive advantage of these animals and their inability to adjust to breeding conditions in Brazil.
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spelling PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannameiLitopenaeus vannameigenetic diversityshrimpVNTRPCRThe genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain could account for the reduced adaptive advantage of these animals and their inability to adjust to breeding conditions in Brazil.Sociedade Brasileira de Genética2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012Genetics and Molecular Biology v.25 n.4 2002reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572002000400012info:eu-repo/semantics/openAccessFreitas,Patrícia Domingues deGaletti Junior,Pedro Manoeleng2003-03-12T00:00:00Zoai:scielo:S1415-47572002000400012Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2003-03-12T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
title PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
spellingShingle PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
Freitas,Patrícia Domingues de
Litopenaeus vannamei
genetic diversity
shrimp
VNTR
PCR
title_short PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
title_full PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
title_fullStr PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
title_full_unstemmed PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
title_sort PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
author Freitas,Patrícia Domingues de
author_facet Freitas,Patrícia Domingues de
Galetti Junior,Pedro Manoel
author_role author
author2 Galetti Junior,Pedro Manoel
author2_role author
dc.contributor.author.fl_str_mv Freitas,Patrícia Domingues de
Galetti Junior,Pedro Manoel
dc.subject.por.fl_str_mv Litopenaeus vannamei
genetic diversity
shrimp
VNTR
PCR
topic Litopenaeus vannamei
genetic diversity
shrimp
VNTR
PCR
description The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain could account for the reduced adaptive advantage of these animals and their inability to adjust to breeding conditions in Brazil.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572002000400012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.25 n.4 2002
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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