R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)

Detalhes bibliográficos
Autor(a) principal: Maistro,Edson Luis
Data de Publicação: 1999
Outros Autores: Foresti,Fausto, Oliveira,Claudio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200011
Resumo: The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other vertebrates.
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spelling R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other vertebrates.Sociedade Brasileira de Genética1999-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200011Genetics and Molecular Biology v.22 n.2 1999reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571999000200011info:eu-repo/semantics/openAccessMaistro,Edson LuisForesti,FaustoOliveira,Claudioeng1999-08-26T00:00:00Zoai:scielo:S1415-47571999000200011Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-08-26T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
title R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
spellingShingle R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
Maistro,Edson Luis
title_short R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
title_full R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
title_fullStr R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
title_full_unstemmed R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
title_sort R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
author Maistro,Edson Luis
author_facet Maistro,Edson Luis
Foresti,Fausto
Oliveira,Claudio
author_role author
author2 Foresti,Fausto
Oliveira,Claudio
author2_role author
author
dc.contributor.author.fl_str_mv Maistro,Edson Luis
Foresti,Fausto
Oliveira,Claudio
description The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other vertebrates.
publishDate 1999
dc.date.none.fl_str_mv 1999-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47571999000200011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.22 n.2 1999
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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instname_str Sociedade Brasileira de Genética (SBG)
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institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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