Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum

Detalhes bibliográficos
Autor(a) principal: Cervelatti,Eliane P.
Data de Publicação: 2004
Outros Autores: Ferreira-Nozawa,Monica S., Aquino-Ferreira,Roseli, Fachin,Ana Lúcia, Martinez-Rossi,Nilce M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100016
Resumo: The electrophoretic karyotype of the dermatophyte Trichophyton rubrum was established using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Five chromosomal bands of approximately 3.0 to 5.8 megabase pairs (Mbp) each were observed and together indicated that 22.05 Mbp of the total genome are organized as chromosomal macromolecules. In addition to establishing the number and size of T. rubrum chromosomes, these results open perspectives for the construction of chromosome-specific libraries and for the physical mapping of genes of interest, thus permitting future gene linkage studies in this pathogen. A detailed understanding of the karyotype and genomic organization of T. rubrum should contribute to further genetic, taxonomic and epidemiological studies of this dermatophyte.
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spelling Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrumTrichophyton rubrumfungal chromosomeselectrophoretic karyotypedermatophytecontour-clamped homogeneous electric fieldThe electrophoretic karyotype of the dermatophyte Trichophyton rubrum was established using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Five chromosomal bands of approximately 3.0 to 5.8 megabase pairs (Mbp) each were observed and together indicated that 22.05 Mbp of the total genome are organized as chromosomal macromolecules. In addition to establishing the number and size of T. rubrum chromosomes, these results open perspectives for the construction of chromosome-specific libraries and for the physical mapping of genes of interest, thus permitting future gene linkage studies in this pathogen. A detailed understanding of the karyotype and genomic organization of T. rubrum should contribute to further genetic, taxonomic and epidemiological studies of this dermatophyte.Sociedade Brasileira de Genética2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100016Genetics and Molecular Biology v.27 n.1 2004reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572004000100016info:eu-repo/semantics/openAccessCervelatti,Eliane P.Ferreira-Nozawa,Monica S.Aquino-Ferreira,RoseliFachin,Ana LúciaMartinez-Rossi,Nilce M.eng2004-04-13T00:00:00Zoai:scielo:S1415-47572004000100016Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2004-04-13T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
title Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
spellingShingle Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
Cervelatti,Eliane P.
Trichophyton rubrum
fungal chromosomes
electrophoretic karyotype
dermatophyte
contour-clamped homogeneous electric field
title_short Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
title_full Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
title_fullStr Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
title_full_unstemmed Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
title_sort Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
author Cervelatti,Eliane P.
author_facet Cervelatti,Eliane P.
Ferreira-Nozawa,Monica S.
Aquino-Ferreira,Roseli
Fachin,Ana Lúcia
Martinez-Rossi,Nilce M.
author_role author
author2 Ferreira-Nozawa,Monica S.
Aquino-Ferreira,Roseli
Fachin,Ana Lúcia
Martinez-Rossi,Nilce M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cervelatti,Eliane P.
Ferreira-Nozawa,Monica S.
Aquino-Ferreira,Roseli
Fachin,Ana Lúcia
Martinez-Rossi,Nilce M.
dc.subject.por.fl_str_mv Trichophyton rubrum
fungal chromosomes
electrophoretic karyotype
dermatophyte
contour-clamped homogeneous electric field
topic Trichophyton rubrum
fungal chromosomes
electrophoretic karyotype
dermatophyte
contour-clamped homogeneous electric field
description The electrophoretic karyotype of the dermatophyte Trichophyton rubrum was established using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Five chromosomal bands of approximately 3.0 to 5.8 megabase pairs (Mbp) each were observed and together indicated that 22.05 Mbp of the total genome are organized as chromosomal macromolecules. In addition to establishing the number and size of T. rubrum chromosomes, these results open perspectives for the construction of chromosome-specific libraries and for the physical mapping of genes of interest, thus permitting future gene linkage studies in this pathogen. A detailed understanding of the karyotype and genomic organization of T. rubrum should contribute to further genetic, taxonomic and epidemiological studies of this dermatophyte.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572004000100016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.27 n.1 2004
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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