First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300026 |
Resumo: | High concentrations of xenobiotics from urban and industrial wastes have contributed to the contamination of many aquatic environments. We used the comet assay to evaluate the genotoxic potential of water collected from the River Paraná, which receives a great deal of waste, at three points (Puerto Piray, Eldorado and Montecarlo) in the Misiones Province of Argentina. The in vivo comet assay used 40 freshwater clams (Corbicula fluminea) while the in vitro comet assay used Chinese hamster (Cricetulus griseus) K1 cell (CHO-K1) cultures with the mutagen ethyl methanesulfonate (EMS) as the positive control and phosphate buffered saline (PBS) as the negative control. Both assays showed statistically significant differences between the three sampling sites in relation to the negative control, the results of this preliminary study indicating that at these three sites water from the Paraná River presents genotoxic potential. |
id |
SBG-1_687930f23a50283131864f5f1346f075 |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572008000300026 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assaybiomonitoringComet assayCorbícula flumineaCHO-K1 cellsgenotoxicityParaná riverHigh concentrations of xenobiotics from urban and industrial wastes have contributed to the contamination of many aquatic environments. We used the comet assay to evaluate the genotoxic potential of water collected from the River Paraná, which receives a great deal of waste, at three points (Puerto Piray, Eldorado and Montecarlo) in the Misiones Province of Argentina. The in vivo comet assay used 40 freshwater clams (Corbicula fluminea) while the in vitro comet assay used Chinese hamster (Cricetulus griseus) K1 cell (CHO-K1) cultures with the mutagen ethyl methanesulfonate (EMS) as the positive control and phosphate buffered saline (PBS) as the negative control. Both assays showed statistically significant differences between the three sampling sites in relation to the negative control, the results of this preliminary study indicating that at these three sites water from the Paraná River presents genotoxic potential.Sociedade Brasileira de Genética2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300026Genetics and Molecular Biology v.31 n.2 2008reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572008000300026info:eu-repo/semantics/openAccessCaffetti,Jacqueline D.Mantovani,Mário S.Pastori,María C.Fenocchio,Alberto S.eng2008-06-24T00:00:00Zoai:scielo:S1415-47572008000300026Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2008-06-24T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay |
title |
First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay |
spellingShingle |
First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay Caffetti,Jacqueline D. biomonitoring Comet assay Corbícula fluminea CHO-K1 cells genotoxicity Paraná river |
title_short |
First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay |
title_full |
First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay |
title_fullStr |
First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay |
title_full_unstemmed |
First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay |
title_sort |
First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay |
author |
Caffetti,Jacqueline D. |
author_facet |
Caffetti,Jacqueline D. Mantovani,Mário S. Pastori,María C. Fenocchio,Alberto S. |
author_role |
author |
author2 |
Mantovani,Mário S. Pastori,María C. Fenocchio,Alberto S. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Caffetti,Jacqueline D. Mantovani,Mário S. Pastori,María C. Fenocchio,Alberto S. |
dc.subject.por.fl_str_mv |
biomonitoring Comet assay Corbícula fluminea CHO-K1 cells genotoxicity Paraná river |
topic |
biomonitoring Comet assay Corbícula fluminea CHO-K1 cells genotoxicity Paraná river |
description |
High concentrations of xenobiotics from urban and industrial wastes have contributed to the contamination of many aquatic environments. We used the comet assay to evaluate the genotoxic potential of water collected from the River Paraná, which receives a great deal of waste, at three points (Puerto Piray, Eldorado and Montecarlo) in the Misiones Province of Argentina. The in vivo comet assay used 40 freshwater clams (Corbicula fluminea) while the in vitro comet assay used Chinese hamster (Cricetulus griseus) K1 cell (CHO-K1) cultures with the mutagen ethyl methanesulfonate (EMS) as the positive control and phosphate buffered saline (PBS) as the negative control. Both assays showed statistically significant differences between the three sampling sites in relation to the negative control, the results of this preliminary study indicating that at these three sites water from the Paraná River presents genotoxic potential. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300026 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300026 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572008000300026 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.31 n.2 2008 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122381186367488 |