DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808 |
Resumo: | Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them. |
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Genetics and Molecular Biology |
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spelling |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian MerganserDNAnoninvasive samplesgenetic diversityendangered birdsMergusAbstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them.Sociedade Brasileira de Genética2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808Genetics and Molecular Biology v.40 n.4 2017reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2016-0297info:eu-repo/semantics/openAccessMaia,Thais AugustaVilaça,Sibelle TorresSilva,Luciana Resende daSantos,Fabricio RodriguesDantas,Gisele Pires de Mendonçaeng2017-11-21T00:00:00Zoai:scielo:S1415-47572017000500808Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2017-11-21T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser |
title |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser |
spellingShingle |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser Maia,Thais Augusta DNA noninvasive samples genetic diversity endangered birds Mergus |
title_short |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser |
title_full |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser |
title_fullStr |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser |
title_full_unstemmed |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser |
title_sort |
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser |
author |
Maia,Thais Augusta |
author_facet |
Maia,Thais Augusta Vilaça,Sibelle Torres Silva,Luciana Resende da Santos,Fabricio Rodrigues Dantas,Gisele Pires de Mendonça |
author_role |
author |
author2 |
Vilaça,Sibelle Torres Silva,Luciana Resende da Santos,Fabricio Rodrigues Dantas,Gisele Pires de Mendonça |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Maia,Thais Augusta Vilaça,Sibelle Torres Silva,Luciana Resende da Santos,Fabricio Rodrigues Dantas,Gisele Pires de Mendonça |
dc.subject.por.fl_str_mv |
DNA noninvasive samples genetic diversity endangered birds Mergus |
topic |
DNA noninvasive samples genetic diversity endangered birds Mergus |
description |
Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2016-0297 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.40 n.4 2017 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122387646644224 |