DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser

Detalhes bibliográficos
Autor(a) principal: Maia,Thais Augusta
Data de Publicação: 2017
Outros Autores: Vilaça,Sibelle Torres, Silva,Luciana Resende da, Santos,Fabricio Rodrigues, Dantas,Gisele Pires de Mendonça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808
Resumo: Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them.
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spelling DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian MerganserDNAnoninvasive samplesgenetic diversityendangered birdsMergusAbstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them.Sociedade Brasileira de Genética2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808Genetics and Molecular Biology v.40 n.4 2017reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2016-0297info:eu-repo/semantics/openAccessMaia,Thais AugustaVilaça,Sibelle TorresSilva,Luciana Resende daSantos,Fabricio RodriguesDantas,Gisele Pires de Mendonçaeng2017-11-21T00:00:00Zoai:scielo:S1415-47572017000500808Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2017-11-21T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
title DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
spellingShingle DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
Maia,Thais Augusta
DNA
noninvasive samples
genetic diversity
endangered birds
Mergus
title_short DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
title_full DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
title_fullStr DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
title_full_unstemmed DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
title_sort DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
author Maia,Thais Augusta
author_facet Maia,Thais Augusta
Vilaça,Sibelle Torres
Silva,Luciana Resende da
Santos,Fabricio Rodrigues
Dantas,Gisele Pires de Mendonça
author_role author
author2 Vilaça,Sibelle Torres
Silva,Luciana Resende da
Santos,Fabricio Rodrigues
Dantas,Gisele Pires de Mendonça
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Maia,Thais Augusta
Vilaça,Sibelle Torres
Silva,Luciana Resende da
Santos,Fabricio Rodrigues
Dantas,Gisele Pires de Mendonça
dc.subject.por.fl_str_mv DNA
noninvasive samples
genetic diversity
endangered birds
Mergus
topic DNA
noninvasive samples
genetic diversity
endangered birds
Mergus
description Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2016-0297
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.40 n.4 2017
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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