Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300025 |
Resumo: | The aim of this research was to study the mating system of a natural population of Theobroma grandiflorum (cupuassu) from Nova Ipixuna, Pará state, using microsatellite markers. Eight polymorphic microsatellite loci were analyzed in eight families, each represented by 10 six-month old seedlings derived from open-pollinated pods. The estimation for the multilocus outcrossing rate (<img border=0 id="_x0000_i1026" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>m = 1.0) and individual outcrossing rate (<img border=0 id="_x0000_i1027" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle> = 1.0) for this population suggests that T. grandiflorum may be a perfect outbreeding (allogamous) species. Likewise, for the studied population the estimate for single locus outcrossing rate (<img border=0 id="_x0000_i1028" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>S) was elevated (0.946), but lower than <img border=0 id="_x0000_i1029" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>m, confirming the likely outcrossing character of the species and suggesting the occurrence of 5.4% biparental inbreeding rate (<img border=0 id="_x0000_i1030" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>m - <img border=0 id="_x0000_i1031" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>S). The estimation of genetic divergence (<img border=0 id="_x0000_i1032" src="../../img/revistas/gmb/v26n3/a25img02.gif" align=absmiddle>st) between allelic frequencies in ovules and pollen revealed a deviation from random mating in 75% of the evaluated loci. Likewise, the estimate of correlation of paternity (<img border=0 id="_x0000_i1033" src="../../img/revistas/gmb/v26n3/a25img03.gif" align=absmiddle>P = 0.930) and the mean coefficient of co-ancestrality within families (<img border=0 id="_x0000_i1034" src="../../img/revistas/gmb/v26n3/a25img04.gif" align=absmiddle>XY = 0.501) indicated that the outcrossings were predominantly correlated, and the offspring were full-sibs. These results suggested that for this particular population of T. grandiflorum, the sampling strategy for genetic conservation and breeding should adopt specific models for families derived from correlated outcrossing (full-sibs) and not the ones usually adopted in classic outcrossing species breeding programs (half-sibs). |
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Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markersAmazoniagermplasmcupuassugenetic conservationsimple sequence repeatThe aim of this research was to study the mating system of a natural population of Theobroma grandiflorum (cupuassu) from Nova Ipixuna, Pará state, using microsatellite markers. Eight polymorphic microsatellite loci were analyzed in eight families, each represented by 10 six-month old seedlings derived from open-pollinated pods. The estimation for the multilocus outcrossing rate (<img border=0 id="_x0000_i1026" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>m = 1.0) and individual outcrossing rate (<img border=0 id="_x0000_i1027" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle> = 1.0) for this population suggests that T. grandiflorum may be a perfect outbreeding (allogamous) species. Likewise, for the studied population the estimate for single locus outcrossing rate (<img border=0 id="_x0000_i1028" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>S) was elevated (0.946), but lower than <img border=0 id="_x0000_i1029" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>m, confirming the likely outcrossing character of the species and suggesting the occurrence of 5.4% biparental inbreeding rate (<img border=0 id="_x0000_i1030" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>m - <img border=0 id="_x0000_i1031" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>S). The estimation of genetic divergence (<img border=0 id="_x0000_i1032" src="../../img/revistas/gmb/v26n3/a25img02.gif" align=absmiddle>st) between allelic frequencies in ovules and pollen revealed a deviation from random mating in 75% of the evaluated loci. Likewise, the estimate of correlation of paternity (<img border=0 id="_x0000_i1033" src="../../img/revistas/gmb/v26n3/a25img03.gif" align=absmiddle>P = 0.930) and the mean coefficient of co-ancestrality within families (<img border=0 id="_x0000_i1034" src="../../img/revistas/gmb/v26n3/a25img04.gif" align=absmiddle>XY = 0.501) indicated that the outcrossings were predominantly correlated, and the offspring were full-sibs. These results suggested that for this particular population of T. grandiflorum, the sampling strategy for genetic conservation and breeding should adopt specific models for families derived from correlated outcrossing (full-sibs) and not the ones usually adopted in classic outcrossing species breeding programs (half-sibs).Sociedade Brasileira de Genética2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300025Genetics and Molecular Biology v.26 n.3 2003reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572003000300025info:eu-repo/semantics/openAccessAlves,Rafael M.Artero,Angela S.Sebbenn,Alexandre M.Figueira,Antonioeng2003-09-29T00:00:00Zoai:scielo:S1415-47572003000300025Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2003-09-29T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers |
title |
Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers |
spellingShingle |
Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers Alves,Rafael M. Amazonia germplasm cupuassu genetic conservation simple sequence repeat |
title_short |
Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers |
title_full |
Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers |
title_fullStr |
Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers |
title_full_unstemmed |
Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers |
title_sort |
Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers |
author |
Alves,Rafael M. |
author_facet |
Alves,Rafael M. Artero,Angela S. Sebbenn,Alexandre M. Figueira,Antonio |
author_role |
author |
author2 |
Artero,Angela S. Sebbenn,Alexandre M. Figueira,Antonio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Alves,Rafael M. Artero,Angela S. Sebbenn,Alexandre M. Figueira,Antonio |
dc.subject.por.fl_str_mv |
Amazonia germplasm cupuassu genetic conservation simple sequence repeat |
topic |
Amazonia germplasm cupuassu genetic conservation simple sequence repeat |
description |
The aim of this research was to study the mating system of a natural population of Theobroma grandiflorum (cupuassu) from Nova Ipixuna, Pará state, using microsatellite markers. Eight polymorphic microsatellite loci were analyzed in eight families, each represented by 10 six-month old seedlings derived from open-pollinated pods. The estimation for the multilocus outcrossing rate (<img border=0 id="_x0000_i1026" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>m = 1.0) and individual outcrossing rate (<img border=0 id="_x0000_i1027" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle> = 1.0) for this population suggests that T. grandiflorum may be a perfect outbreeding (allogamous) species. Likewise, for the studied population the estimate for single locus outcrossing rate (<img border=0 id="_x0000_i1028" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>S) was elevated (0.946), but lower than <img border=0 id="_x0000_i1029" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>m, confirming the likely outcrossing character of the species and suggesting the occurrence of 5.4% biparental inbreeding rate (<img border=0 id="_x0000_i1030" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>m - <img border=0 id="_x0000_i1031" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle>S). The estimation of genetic divergence (<img border=0 id="_x0000_i1032" src="../../img/revistas/gmb/v26n3/a25img02.gif" align=absmiddle>st) between allelic frequencies in ovules and pollen revealed a deviation from random mating in 75% of the evaluated loci. Likewise, the estimate of correlation of paternity (<img border=0 id="_x0000_i1033" src="../../img/revistas/gmb/v26n3/a25img03.gif" align=absmiddle>P = 0.930) and the mean coefficient of co-ancestrality within families (<img border=0 id="_x0000_i1034" src="../../img/revistas/gmb/v26n3/a25img04.gif" align=absmiddle>XY = 0.501) indicated that the outcrossings were predominantly correlated, and the offspring were full-sibs. These results suggested that for this particular population of T. grandiflorum, the sampling strategy for genetic conservation and breeding should adopt specific models for families derived from correlated outcrossing (full-sibs) and not the ones usually adopted in classic outcrossing species breeding programs (half-sibs). |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300025 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572003000300025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.26 n.3 2003 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122378675027968 |