Estimating genomic instability mediated by Alu retroelements in breast cancer
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100004 |
Resumo: | Alu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of well-known genomic instability in breast and other types of cancer. These results indicate the potential usefulness of Alu-PCR in identifying altered gene sequences in breast cancer. However, caution is required in its application since the Alu primer can produce non-specific amplification. |
id |
SBG-1_797685253751a16280246ecbb490a5f0 |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572009000100004 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
Estimating genomic instability mediated by Alu retroelements in breast cancerAlu-PCRbreast cancergene insertionsgene deletionsinvasive ductal carcinomarecombinationAlu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of well-known genomic instability in breast and other types of cancer. These results indicate the potential usefulness of Alu-PCR in identifying altered gene sequences in breast cancer. However, caution is required in its application since the Alu primer can produce non-specific amplification.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100004Genetics and Molecular Biology v.32 n.1 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000018info:eu-repo/semantics/openAccessFazza,Ana CristinaSabino,Flavia CalSetta,Nathalia deBordin Jr.,Newton AntonioSilva,Eloiza Helena Tajara daCarareto,Claudia Marcia Aparecidaeng2009-02-10T00:00:00Zoai:scielo:S1415-47572009000100004Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-02-10T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Estimating genomic instability mediated by Alu retroelements in breast cancer |
title |
Estimating genomic instability mediated by Alu retroelements in breast cancer |
spellingShingle |
Estimating genomic instability mediated by Alu retroelements in breast cancer Fazza,Ana Cristina Alu-PCR breast cancer gene insertions gene deletions invasive ductal carcinoma recombination |
title_short |
Estimating genomic instability mediated by Alu retroelements in breast cancer |
title_full |
Estimating genomic instability mediated by Alu retroelements in breast cancer |
title_fullStr |
Estimating genomic instability mediated by Alu retroelements in breast cancer |
title_full_unstemmed |
Estimating genomic instability mediated by Alu retroelements in breast cancer |
title_sort |
Estimating genomic instability mediated by Alu retroelements in breast cancer |
author |
Fazza,Ana Cristina |
author_facet |
Fazza,Ana Cristina Sabino,Flavia Cal Setta,Nathalia de Bordin Jr.,Newton Antonio Silva,Eloiza Helena Tajara da Carareto,Claudia Marcia Aparecida |
author_role |
author |
author2 |
Sabino,Flavia Cal Setta,Nathalia de Bordin Jr.,Newton Antonio Silva,Eloiza Helena Tajara da Carareto,Claudia Marcia Aparecida |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fazza,Ana Cristina Sabino,Flavia Cal Setta,Nathalia de Bordin Jr.,Newton Antonio Silva,Eloiza Helena Tajara da Carareto,Claudia Marcia Aparecida |
dc.subject.por.fl_str_mv |
Alu-PCR breast cancer gene insertions gene deletions invasive ductal carcinoma recombination |
topic |
Alu-PCR breast cancer gene insertions gene deletions invasive ductal carcinoma recombination |
description |
Alu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of well-known genomic instability in breast and other types of cancer. These results indicate the potential usefulness of Alu-PCR in identifying altered gene sequences in breast cancer. However, caution is required in its application since the Alu primer can produce non-specific amplification. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572009005000018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.32 n.1 2009 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122382019985408 |