Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells

Detalhes bibliográficos
Autor(a) principal: Drets,Máximo E.
Data de Publicação: 1998
Outros Autores: Mendizábal,Marcos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200008
Resumo: Microphotometrical scanning and computer graphic image analysis were carried out to detect the distribution of chromatin densities in subtelomeric segments of T-banded endoreduplicated chromosomes of Chinese hamster ovary (CHO) cells. Chromatin density patterns detected with this method were similar to those previously found in CHO and normal human chromosomes. The highest chromatin densities were considered as marker segments which led to the detection of reciprocal changes of position in endoreduplicated chromosomes during cell spreading on the slide. The problem of the subtelomeric T-banding density patterns found in the endoreduplicated chromosomes and their relation to the structure and molecular composition of this region is briefly discussed.
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spelling Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cellsMicrophotometrical scanning and computer graphic image analysis were carried out to detect the distribution of chromatin densities in subtelomeric segments of T-banded endoreduplicated chromosomes of Chinese hamster ovary (CHO) cells. Chromatin density patterns detected with this method were similar to those previously found in CHO and normal human chromosomes. The highest chromatin densities were considered as marker segments which led to the detection of reciprocal changes of position in endoreduplicated chromosomes during cell spreading on the slide. The problem of the subtelomeric T-banding density patterns found in the endoreduplicated chromosomes and their relation to the structure and molecular composition of this region is briefly discussed.Sociedade Brasileira de Genética1998-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200008Genetics and Molecular Biology v.21 n.2 1998reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571998000200008info:eu-repo/semantics/openAccessDrets,Máximo E.Mendizábal,Marcoseng1999-01-06T00:00:00Zoai:scielo:S1415-47571998000200008Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-01-06T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells
title Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells
spellingShingle Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells
Drets,Máximo E.
title_short Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells
title_full Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells
title_fullStr Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells
title_full_unstemmed Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells
title_sort Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells
author Drets,Máximo E.
author_facet Drets,Máximo E.
Mendizábal,Marcos
author_role author
author2 Mendizábal,Marcos
author2_role author
dc.contributor.author.fl_str_mv Drets,Máximo E.
Mendizábal,Marcos
description Microphotometrical scanning and computer graphic image analysis were carried out to detect the distribution of chromatin densities in subtelomeric segments of T-banded endoreduplicated chromosomes of Chinese hamster ovary (CHO) cells. Chromatin density patterns detected with this method were similar to those previously found in CHO and normal human chromosomes. The highest chromatin densities were considered as marker segments which led to the detection of reciprocal changes of position in endoreduplicated chromosomes during cell spreading on the slide. The problem of the subtelomeric T-banding density patterns found in the endoreduplicated chromosomes and their relation to the structure and molecular composition of this region is briefly discussed.
publishDate 1998
dc.date.none.fl_str_mv 1998-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47571998000200008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.21 n.2 1998
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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instname_str Sociedade Brasileira de Genética (SBG)
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institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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