Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021 |
Resumo: | In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus. |
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Genetics and Molecular Biology |
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Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus EpidendrumEpidendrumOrchidaceaeshort tandem repeatcross-amplificationIn this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021Genetics and Molecular Biology v.32 n.2 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000037info:eu-repo/semantics/openAccessPinheiro,FábioPalma-Silva,ClarisseBarros,Fábio deCozzolino,Salvatoreeng2009-05-25T00:00:00Zoai:scielo:S1415-47572009000200021Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-05-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum |
title |
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum |
spellingShingle |
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum Pinheiro,Fábio Epidendrum Orchidaceae short tandem repeat cross-amplification |
title_short |
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum |
title_full |
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum |
title_fullStr |
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum |
title_full_unstemmed |
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum |
title_sort |
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum |
author |
Pinheiro,Fábio |
author_facet |
Pinheiro,Fábio Palma-Silva,Clarisse Barros,Fábio de Cozzolino,Salvatore |
author_role |
author |
author2 |
Palma-Silva,Clarisse Barros,Fábio de Cozzolino,Salvatore |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pinheiro,Fábio Palma-Silva,Clarisse Barros,Fábio de Cozzolino,Salvatore |
dc.subject.por.fl_str_mv |
Epidendrum Orchidaceae short tandem repeat cross-amplification |
topic |
Epidendrum Orchidaceae short tandem repeat cross-amplification |
description |
In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572009005000037 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.32 n.2 2009 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122382297858048 |