Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum

Detalhes bibliográficos
Autor(a) principal: Pinheiro,Fábio
Data de Publicação: 2009
Outros Autores: Palma-Silva,Clarisse, Barros,Fábio de, Cozzolino,Salvatore
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021
Resumo: In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.
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spelling Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus EpidendrumEpidendrumOrchidaceaeshort tandem repeatcross-amplificationIn this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021Genetics and Molecular Biology v.32 n.2 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000037info:eu-repo/semantics/openAccessPinheiro,FábioPalma-Silva,ClarisseBarros,Fábio deCozzolino,Salvatoreeng2009-05-25T00:00:00Zoai:scielo:S1415-47572009000200021Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-05-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
title Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
spellingShingle Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
Pinheiro,Fábio
Epidendrum
Orchidaceae
short tandem repeat
cross-amplification
title_short Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
title_full Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
title_fullStr Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
title_full_unstemmed Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
title_sort Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
author Pinheiro,Fábio
author_facet Pinheiro,Fábio
Palma-Silva,Clarisse
Barros,Fábio de
Cozzolino,Salvatore
author_role author
author2 Palma-Silva,Clarisse
Barros,Fábio de
Cozzolino,Salvatore
author2_role author
author
author
dc.contributor.author.fl_str_mv Pinheiro,Fábio
Palma-Silva,Clarisse
Barros,Fábio de
Cozzolino,Salvatore
dc.subject.por.fl_str_mv Epidendrum
Orchidaceae
short tandem repeat
cross-amplification
topic Epidendrum
Orchidaceae
short tandem repeat
cross-amplification
description In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572009005000037
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.32 n.2 2009
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
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institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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