Genetic machinery for RNA silencing and defense against viruses in Citrus

Detalhes bibliográficos
Autor(a) principal: Benedito,Vagner Augusto
Data de Publicação: 2007
Outros Autores: Faria,Laura, Freitas-Astúa,Juliana, Figueira,Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500027
Resumo: RNA silencing mechanisms are conserved throughout eukaryotic evolution, possibly due to their importance in viral resistance and other aspects of cell biology. Here, we explored the Citrus EST (CitEST) database in search of sequences related to the most important known genes involved in RNA silencing. Transcripts strongly matching Argonaute (AGO), Dicer-like (DCL), Hua enhancer (HEN), and RNA-dependent RNA Polymerase (RdRP) were found in many of the citrus libraries. The reads were clustered and quantified. This shows that post-transcriptional gene silencing apparatus is active in citrus. It seems plausible that a better understanding of the players of RNA silencing in Citrus spp. and related genera may help create new tools to defeat the viral diseases that affect the citrus industry. Functional analyses of these citrus genes would enable the pursuit of this hypothesis.
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spelling Genetic machinery for RNA silencing and defense against viruses in CitrusCitESTGene silencingPTGSRNA silencing mechanisms are conserved throughout eukaryotic evolution, possibly due to their importance in viral resistance and other aspects of cell biology. Here, we explored the Citrus EST (CitEST) database in search of sequences related to the most important known genes involved in RNA silencing. Transcripts strongly matching Argonaute (AGO), Dicer-like (DCL), Hua enhancer (HEN), and RNA-dependent RNA Polymerase (RdRP) were found in many of the citrus libraries. The reads were clustered and quantified. This shows that post-transcriptional gene silencing apparatus is active in citrus. It seems plausible that a better understanding of the players of RNA silencing in Citrus spp. and related genera may help create new tools to defeat the viral diseases that affect the citrus industry. Functional analyses of these citrus genes would enable the pursuit of this hypothesis.Sociedade Brasileira de Genética2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500027Genetics and Molecular Biology v.30 n.3 suppl.0 2007reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572007000500027info:eu-repo/semantics/openAccessBenedito,Vagner AugustoFaria,LauraFreitas-Astúa,JulianaFigueira,Antonioeng2007-11-06T00:00:00Zoai:scielo:S1415-47572007000500027Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2007-11-06T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Genetic machinery for RNA silencing and defense against viruses in Citrus
title Genetic machinery for RNA silencing and defense against viruses in Citrus
spellingShingle Genetic machinery for RNA silencing and defense against viruses in Citrus
Benedito,Vagner Augusto
CitEST
Gene silencing
PTGS
title_short Genetic machinery for RNA silencing and defense against viruses in Citrus
title_full Genetic machinery for RNA silencing and defense against viruses in Citrus
title_fullStr Genetic machinery for RNA silencing and defense against viruses in Citrus
title_full_unstemmed Genetic machinery for RNA silencing and defense against viruses in Citrus
title_sort Genetic machinery for RNA silencing and defense against viruses in Citrus
author Benedito,Vagner Augusto
author_facet Benedito,Vagner Augusto
Faria,Laura
Freitas-Astúa,Juliana
Figueira,Antonio
author_role author
author2 Faria,Laura
Freitas-Astúa,Juliana
Figueira,Antonio
author2_role author
author
author
dc.contributor.author.fl_str_mv Benedito,Vagner Augusto
Faria,Laura
Freitas-Astúa,Juliana
Figueira,Antonio
dc.subject.por.fl_str_mv CitEST
Gene silencing
PTGS
topic CitEST
Gene silencing
PTGS
description RNA silencing mechanisms are conserved throughout eukaryotic evolution, possibly due to their importance in viral resistance and other aspects of cell biology. Here, we explored the Citrus EST (CitEST) database in search of sequences related to the most important known genes involved in RNA silencing. Transcripts strongly matching Argonaute (AGO), Dicer-like (DCL), Hua enhancer (HEN), and RNA-dependent RNA Polymerase (RdRP) were found in many of the citrus libraries. The reads were clustered and quantified. This shows that post-transcriptional gene silencing apparatus is active in citrus. It seems plausible that a better understanding of the players of RNA silencing in Citrus spp. and related genera may help create new tools to defeat the viral diseases that affect the citrus industry. Functional analyses of these citrus genes would enable the pursuit of this hypothesis.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500027
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572007000500027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.30 n.3 suppl.0 2007
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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instname_str Sociedade Brasileira de Genética (SBG)
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institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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