Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)

Detalhes bibliográficos
Autor(a) principal: Silva,Guilherme Messias da
Data de Publicação: 2009
Outros Autores: Bione,Edgar Guimarães, Cabral-de-Mello,Diogo Cavalcanti, Moura,Rita de Cássia de, Simões,Zilá Luz Paulino, Souza,Maria José de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012
Resumo: Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.
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spelling Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)chromosome rearrangementsheterochromatinkaryotypeNORsFISHMeiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012Genetics and Molecular Biology v.32 n.2 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000040info:eu-repo/semantics/openAccessSilva,Guilherme Messias daBione,Edgar GuimarãesCabral-de-Mello,Diogo CavalcantiMoura,Rita de Cássia deSimões,Zilá Luz PaulinoSouza,Maria José deeng2009-05-25T00:00:00Zoai:scielo:S1415-47572009000200012Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-05-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
spellingShingle Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
Silva,Guilherme Messias da
chromosome rearrangements
heterochromatin
karyotype
NORs
FISH
title_short Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title_full Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title_fullStr Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title_full_unstemmed Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title_sort Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
author Silva,Guilherme Messias da
author_facet Silva,Guilherme Messias da
Bione,Edgar Guimarães
Cabral-de-Mello,Diogo Cavalcanti
Moura,Rita de Cássia de
Simões,Zilá Luz Paulino
Souza,Maria José de
author_role author
author2 Bione,Edgar Guimarães
Cabral-de-Mello,Diogo Cavalcanti
Moura,Rita de Cássia de
Simões,Zilá Luz Paulino
Souza,Maria José de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Guilherme Messias da
Bione,Edgar Guimarães
Cabral-de-Mello,Diogo Cavalcanti
Moura,Rita de Cássia de
Simões,Zilá Luz Paulino
Souza,Maria José de
dc.subject.por.fl_str_mv chromosome rearrangements
heterochromatin
karyotype
NORs
FISH
topic chromosome rearrangements
heterochromatin
karyotype
NORs
FISH
description Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572009005000040
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.32 n.2 2009
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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