Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae)
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200014 |
Resumo: | Rhynchospora tenuis Link (Cyperaceae) is a weed widely distributed in Brazil that presents a small number of holocentric chromosomes (2n = 4) with some autopolyploid populations (2n = 8). The haploid number n = 2 is considered as a derivative of the base number x = 5. 45S rDNA probes and telomeric DNA were hybridized in both chromosome races of R. tenuis, looking for indications of chromosome fusions. The results showed that hybridization sites of the telomeric probe were restricted to end chromosome regions whereas rDNA sites were terminally located. The chromosome race with n = 4 exhibited a doubled number of sites, with similar size and location to the hybridized sequences, confirming its autopolyploid origin. Furthermore, the terminal location of the single 45S rDNA site in the 2n = 4 race suggested that disploid reduction in Rhynchospora, from n = 5 to n = 2, was followed by elimination or reorganization events, keeping the terminal distribution of these sites, as in an others species of the genus. |
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Genetics and Molecular Biology |
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Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae)CyperaceaeFISHholocentric chromosomes45S rDNAtelomere sequencesRhynchospora tenuis Link (Cyperaceae) is a weed widely distributed in Brazil that presents a small number of holocentric chromosomes (2n = 4) with some autopolyploid populations (2n = 8). The haploid number n = 2 is considered as a derivative of the base number x = 5. 45S rDNA probes and telomeric DNA were hybridized in both chromosome races of R. tenuis, looking for indications of chromosome fusions. The results showed that hybridization sites of the telomeric probe were restricted to end chromosome regions whereas rDNA sites were terminally located. The chromosome race with n = 4 exhibited a doubled number of sites, with similar size and location to the hybridized sequences, confirming its autopolyploid origin. Furthermore, the terminal location of the single 45S rDNA site in the 2n = 4 race suggested that disploid reduction in Rhynchospora, from n = 5 to n = 2, was followed by elimination or reorganization events, keeping the terminal distribution of these sites, as in an others species of the genus.Sociedade Brasileira de Genética2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200014Genetics and Molecular Biology v.26 n.2 2003reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572003000200014info:eu-repo/semantics/openAccessVanzela,André L.L.Cuadrado,AngelesGuerra,Marceloeng2003-06-25T00:00:00Zoai:scielo:S1415-47572003000200014Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2003-06-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae) |
title |
Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae) |
spellingShingle |
Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae) Vanzela,André L.L. Cyperaceae FISH holocentric chromosomes 45S rDNA telomere sequences |
title_short |
Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae) |
title_full |
Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae) |
title_fullStr |
Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae) |
title_full_unstemmed |
Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae) |
title_sort |
Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae) |
author |
Vanzela,André L.L. |
author_facet |
Vanzela,André L.L. Cuadrado,Angeles Guerra,Marcelo |
author_role |
author |
author2 |
Cuadrado,Angeles Guerra,Marcelo |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Vanzela,André L.L. Cuadrado,Angeles Guerra,Marcelo |
dc.subject.por.fl_str_mv |
Cyperaceae FISH holocentric chromosomes 45S rDNA telomere sequences |
topic |
Cyperaceae FISH holocentric chromosomes 45S rDNA telomere sequences |
description |
Rhynchospora tenuis Link (Cyperaceae) is a weed widely distributed in Brazil that presents a small number of holocentric chromosomes (2n = 4) with some autopolyploid populations (2n = 8). The haploid number n = 2 is considered as a derivative of the base number x = 5. 45S rDNA probes and telomeric DNA were hybridized in both chromosome races of R. tenuis, looking for indications of chromosome fusions. The results showed that hybridization sites of the telomeric probe were restricted to end chromosome regions whereas rDNA sites were terminally located. The chromosome race with n = 4 exhibited a doubled number of sites, with similar size and location to the hybridized sequences, confirming its autopolyploid origin. Furthermore, the terminal location of the single 45S rDNA site in the 2n = 4 race suggested that disploid reduction in Rhynchospora, from n = 5 to n = 2, was followed by elimination or reorganization events, keeping the terminal distribution of these sites, as in an others species of the genus. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572003000200014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.26 n.2 2003 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122378634133504 |