Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder

Detalhes bibliográficos
Autor(a) principal: Pljesa-Ercegovac,Marija
Data de Publicação: 2010
Outros Autores: Savic-Radojevic,Ana, Kravic-Stevovic,Tamara, Bumbasirevic,Vladimir, Mimic-Oka,Jasmina, Simic,Tatiana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300013
Resumo: Transitional cell carcinoma (TCC) of urinary bladder belongs to glutathione S-transferase P1 (GSTP1) overexpressing tumors. Upregulated GSTP1 in TCC is related to apoptosis inhibition. This antiapoptotic effects of GSTP1 might be mediated through protein:protein interaction with c-Jun NH2-terminal kinase (JNK). Herein, we analyzed whether a direct link between GSTP1 and JNK exists in TCC. The presence of GSTP1/JNK complexes was analyzed by immunoprecipitation and Western blotting in 20 TCC specimens, obtained after surgery. Co-localization of GSTP1 and JNK was also investigated in the 5637 TCC cell line by immunofluorescence confocal microscopy. By means of immunoprecipitation we show for the first time the presence of GSTP1/JNK complexes in all TCC samples studied. A co-localization of GSTP1 and JNK was also demonstrated in the 5637 TCC cell line by means of confocal microscopy. Protein-protein interactions, together with co-localization between GSTP1 and JNK provide evidence that GSTP1 most probably inhibits apoptosis in TCC cells by non-covalent binding to JNK.
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spelling Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladderglutathione S-transferaseJNKTCCco-localizationTransitional cell carcinoma (TCC) of urinary bladder belongs to glutathione S-transferase P1 (GSTP1) overexpressing tumors. Upregulated GSTP1 in TCC is related to apoptosis inhibition. This antiapoptotic effects of GSTP1 might be mediated through protein:protein interaction with c-Jun NH2-terminal kinase (JNK). Herein, we analyzed whether a direct link between GSTP1 and JNK exists in TCC. The presence of GSTP1/JNK complexes was analyzed by immunoprecipitation and Western blotting in 20 TCC specimens, obtained after surgery. Co-localization of GSTP1 and JNK was also investigated in the 5637 TCC cell line by immunofluorescence confocal microscopy. By means of immunoprecipitation we show for the first time the presence of GSTP1/JNK complexes in all TCC samples studied. A co-localization of GSTP1 and JNK was also demonstrated in the 5637 TCC cell line by means of confocal microscopy. Protein-protein interactions, together with co-localization between GSTP1 and JNK provide evidence that GSTP1 most probably inhibits apoptosis in TCC cells by non-covalent binding to JNK.Sociedade Brasileira de Genética2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300013Genetics and Molecular Biology v.33 n.3 2010reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572010005000063info:eu-repo/semantics/openAccessPljesa-Ercegovac,MarijaSavic-Radojevic,AnaKravic-Stevovic,TamaraBumbasirevic,VladimirMimic-Oka,JasminaSimic,Tatianaeng2010-08-18T00:00:00Zoai:scielo:S1415-47572010000300013Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2010-08-18T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
title Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
spellingShingle Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
Pljesa-Ercegovac,Marija
glutathione S-transferase
JNK
TCC
co-localization
title_short Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
title_full Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
title_fullStr Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
title_full_unstemmed Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
title_sort Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
author Pljesa-Ercegovac,Marija
author_facet Pljesa-Ercegovac,Marija
Savic-Radojevic,Ana
Kravic-Stevovic,Tamara
Bumbasirevic,Vladimir
Mimic-Oka,Jasmina
Simic,Tatiana
author_role author
author2 Savic-Radojevic,Ana
Kravic-Stevovic,Tamara
Bumbasirevic,Vladimir
Mimic-Oka,Jasmina
Simic,Tatiana
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pljesa-Ercegovac,Marija
Savic-Radojevic,Ana
Kravic-Stevovic,Tamara
Bumbasirevic,Vladimir
Mimic-Oka,Jasmina
Simic,Tatiana
dc.subject.por.fl_str_mv glutathione S-transferase
JNK
TCC
co-localization
topic glutathione S-transferase
JNK
TCC
co-localization
description Transitional cell carcinoma (TCC) of urinary bladder belongs to glutathione S-transferase P1 (GSTP1) overexpressing tumors. Upregulated GSTP1 in TCC is related to apoptosis inhibition. This antiapoptotic effects of GSTP1 might be mediated through protein:protein interaction with c-Jun NH2-terminal kinase (JNK). Herein, we analyzed whether a direct link between GSTP1 and JNK exists in TCC. The presence of GSTP1/JNK complexes was analyzed by immunoprecipitation and Western blotting in 20 TCC specimens, obtained after surgery. Co-localization of GSTP1 and JNK was also investigated in the 5637 TCC cell line by immunofluorescence confocal microscopy. By means of immunoprecipitation we show for the first time the presence of GSTP1/JNK complexes in all TCC samples studied. A co-localization of GSTP1 and JNK was also demonstrated in the 5637 TCC cell line by means of confocal microscopy. Protein-protein interactions, together with co-localization between GSTP1 and JNK provide evidence that GSTP1 most probably inhibits apoptosis in TCC cells by non-covalent binding to JNK.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572010005000063
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.33 n.3 2010
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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