Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100025 |
Resumo: | The Gram-negative bacterium Xanthomonas axonopodis pv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xac capacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodis pv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsD gene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodis pv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole. |
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Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulencecitrus cankertransposometype II secretion systemThe Gram-negative bacterium Xanthomonas axonopodis pv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xac capacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodis pv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsD gene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodis pv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole.Sociedade Brasileira de Genética2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100025Genetics and Molecular Biology v.33 n.1 2010reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000110info:eu-repo/semantics/openAccessBaptista,Juliana CristinaMachado,Marcos AntonioHomem,Rafael AugustoTorres,Pablo SebastiánVojnov,Adrian AlbertoAmaral,Alexandre Morais doeng2010-02-12T00:00:00Zoai:scielo:S1415-47572010000100025Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2010-02-12T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
spellingShingle |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence Baptista,Juliana Cristina citrus canker transposome type II secretion system |
title_short |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title_full |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title_fullStr |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title_full_unstemmed |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title_sort |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
author |
Baptista,Juliana Cristina |
author_facet |
Baptista,Juliana Cristina Machado,Marcos Antonio Homem,Rafael Augusto Torres,Pablo Sebastián Vojnov,Adrian Alberto Amaral,Alexandre Morais do |
author_role |
author |
author2 |
Machado,Marcos Antonio Homem,Rafael Augusto Torres,Pablo Sebastián Vojnov,Adrian Alberto Amaral,Alexandre Morais do |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Baptista,Juliana Cristina Machado,Marcos Antonio Homem,Rafael Augusto Torres,Pablo Sebastián Vojnov,Adrian Alberto Amaral,Alexandre Morais do |
dc.subject.por.fl_str_mv |
citrus canker transposome type II secretion system |
topic |
citrus canker transposome type II secretion system |
description |
The Gram-negative bacterium Xanthomonas axonopodis pv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xac capacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodis pv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsD gene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodis pv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100025 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572009005000110 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.33 n.1 2010 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122382712045568 |