Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil

Detalhes bibliográficos
Autor(a) principal: Silva,Elizabete Cristina da
Data de Publicação: 2014
Outros Autores: McManus,Concepta Margaret, Guimarães,Maria Pia Souza Lima Mattos de Paiva, Gouveia,Aurora M.G., Facó,Olivardo, Pimentel,Daniel M., Caetano,Alexandre Rodrigues, Paiva,Samuel Rezende
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010
Resumo: Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided.
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spelling Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazilparentage errorsanimal breedinganimal genetic resourcesCapra hircusBrazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided.Sociedade Brasileira de Genética2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010Genetics and Molecular Biology v.37 n.1 2014reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572014000100010info:eu-repo/semantics/openAccessSilva,Elizabete Cristina daMcManus,Concepta MargaretGuimarães,Maria Pia Souza Lima Mattos de PaivaGouveia,Aurora M.G.Facó,OlivardoPimentel,Daniel M.Caetano,Alexandre RodriguesPaiva,Samuel Rezendeeng2014-03-24T00:00:00Zoai:scielo:S1415-47572014000100010Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2014-03-24T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
title Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
spellingShingle Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
Silva,Elizabete Cristina da
parentage errors
animal breeding
animal genetic resources
Capra hircus
title_short Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
title_full Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
title_fullStr Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
title_full_unstemmed Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
title_sort Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
author Silva,Elizabete Cristina da
author_facet Silva,Elizabete Cristina da
McManus,Concepta Margaret
Guimarães,Maria Pia Souza Lima Mattos de Paiva
Gouveia,Aurora M.G.
Facó,Olivardo
Pimentel,Daniel M.
Caetano,Alexandre Rodrigues
Paiva,Samuel Rezende
author_role author
author2 McManus,Concepta Margaret
Guimarães,Maria Pia Souza Lima Mattos de Paiva
Gouveia,Aurora M.G.
Facó,Olivardo
Pimentel,Daniel M.
Caetano,Alexandre Rodrigues
Paiva,Samuel Rezende
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Elizabete Cristina da
McManus,Concepta Margaret
Guimarães,Maria Pia Souza Lima Mattos de Paiva
Gouveia,Aurora M.G.
Facó,Olivardo
Pimentel,Daniel M.
Caetano,Alexandre Rodrigues
Paiva,Samuel Rezende
dc.subject.por.fl_str_mv parentage errors
animal breeding
animal genetic resources
Capra hircus
topic parentage errors
animal breeding
animal genetic resources
Capra hircus
description Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572014000100010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.37 n.1 2014
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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