Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
Main Author: | |
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Publication Date: | 2014 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Genetics and Molecular Biology |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010 |
Summary: | Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided. |
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Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazilparentage errorsanimal breedinganimal genetic resourcesCapra hircusBrazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided.Sociedade Brasileira de Genética2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010Genetics and Molecular Biology v.37 n.1 2014reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572014000100010info:eu-repo/semantics/openAccessSilva,Elizabete Cristina daMcManus,Concepta MargaretGuimarães,Maria Pia Souza Lima Mattos de PaivaGouveia,Aurora M.G.Facó,OlivardoPimentel,Daniel M.Caetano,Alexandre RodriguesPaiva,Samuel Rezendeeng2014-03-24T00:00:00Zoai:scielo:S1415-47572014000100010Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2014-03-24T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
spellingShingle |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil Silva,Elizabete Cristina da parentage errors animal breeding animal genetic resources Capra hircus |
title_short |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_full |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_fullStr |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_full_unstemmed |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_sort |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
author |
Silva,Elizabete Cristina da |
author_facet |
Silva,Elizabete Cristina da McManus,Concepta Margaret Guimarães,Maria Pia Souza Lima Mattos de Paiva Gouveia,Aurora M.G. Facó,Olivardo Pimentel,Daniel M. Caetano,Alexandre Rodrigues Paiva,Samuel Rezende |
author_role |
author |
author2 |
McManus,Concepta Margaret Guimarães,Maria Pia Souza Lima Mattos de Paiva Gouveia,Aurora M.G. Facó,Olivardo Pimentel,Daniel M. Caetano,Alexandre Rodrigues Paiva,Samuel Rezende |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Elizabete Cristina da McManus,Concepta Margaret Guimarães,Maria Pia Souza Lima Mattos de Paiva Gouveia,Aurora M.G. Facó,Olivardo Pimentel,Daniel M. Caetano,Alexandre Rodrigues Paiva,Samuel Rezende |
dc.subject.por.fl_str_mv |
parentage errors animal breeding animal genetic resources Capra hircus |
topic |
parentage errors animal breeding animal genetic resources Capra hircus |
description |
Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572014000100010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.37 n.1 2014 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122385826316288 |