Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD

Detalhes bibliográficos
Autor(a) principal: Bastianel,Marinês
Data de Publicação: 1998
Outros Autores: Schwarz,Sérgio F., Coleta Filho,Helvécio Della, Lin,Linda Lee, Machado,Marcos, Koller,Otto C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100020
Resumo: The randomly amplified polymorphic DNA (RAPD) technique was used to distinguish nucellar and zygotic seedlings resulting from crosses between the ‘Montenegrina’ (Citrus deliciosa Tenore) and‘ King’ (C. nobilis Loureiro) tangerines. The aim of the present study was to develop tangerine varieties with a reduced number of seeds and organoleptic characteristics similar to the ‘Montenegrina’ tangerine. Embryos were isolated from seeds, cultivated in vitro, and acclimated in a greenhouse. Four random primers were used to identify 54 plants of sexual origin from a total of 202 individuals. The degree of polymorphism of each primer was reflected in the number of zygotic plants obtained per primer. Cluster analysis of parents and progeny separated the individuals into distinct groups with a maximum genetic dissimilarity of 20%.
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spelling Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPDThe randomly amplified polymorphic DNA (RAPD) technique was used to distinguish nucellar and zygotic seedlings resulting from crosses between the ‘Montenegrina’ (Citrus deliciosa Tenore) and‘ King’ (C. nobilis Loureiro) tangerines. The aim of the present study was to develop tangerine varieties with a reduced number of seeds and organoleptic characteristics similar to the ‘Montenegrina’ tangerine. Embryos were isolated from seeds, cultivated in vitro, and acclimated in a greenhouse. Four random primers were used to identify 54 plants of sexual origin from a total of 202 individuals. The degree of polymorphism of each primer was reflected in the number of zygotic plants obtained per primer. Cluster analysis of parents and progeny separated the individuals into distinct groups with a maximum genetic dissimilarity of 20%.Sociedade Brasileira de Genética1998-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100020Genetics and Molecular Biology v.21 n.1 1998reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571998000100020info:eu-repo/semantics/openAccessBastianel,MarinêsSchwarz,Sérgio F.Coleta Filho,Helvécio DellaLin,Linda LeeMachado,MarcosKoller,Otto C.eng1999-01-06T00:00:00Zoai:scielo:S1415-47571998000100020Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-01-06T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD
title Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD
spellingShingle Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD
Bastianel,Marinês
title_short Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD
title_full Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD
title_fullStr Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD
title_full_unstemmed Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD
title_sort Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD
author Bastianel,Marinês
author_facet Bastianel,Marinês
Schwarz,Sérgio F.
Coleta Filho,Helvécio Della
Lin,Linda Lee
Machado,Marcos
Koller,Otto C.
author_role author
author2 Schwarz,Sérgio F.
Coleta Filho,Helvécio Della
Lin,Linda Lee
Machado,Marcos
Koller,Otto C.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bastianel,Marinês
Schwarz,Sérgio F.
Coleta Filho,Helvécio Della
Lin,Linda Lee
Machado,Marcos
Koller,Otto C.
description The randomly amplified polymorphic DNA (RAPD) technique was used to distinguish nucellar and zygotic seedlings resulting from crosses between the ‘Montenegrina’ (Citrus deliciosa Tenore) and‘ King’ (C. nobilis Loureiro) tangerines. The aim of the present study was to develop tangerine varieties with a reduced number of seeds and organoleptic characteristics similar to the ‘Montenegrina’ tangerine. Embryos were isolated from seeds, cultivated in vitro, and acclimated in a greenhouse. Four random primers were used to identify 54 plants of sexual origin from a total of 202 individuals. The degree of polymorphism of each primer was reflected in the number of zygotic plants obtained per primer. Cluster analysis of parents and progeny separated the individuals into distinct groups with a maximum genetic dissimilarity of 20%.
publishDate 1998
dc.date.none.fl_str_mv 1998-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47571998000100020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.21 n.1 1998
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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