Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500103 |
Resumo: | Abstract Psoriasis is a common chronic, immune-mediated inflammatory disease of the skin. PSORS1C3 is a non-protein coding gene, of which the RNA transcript is found in psoriatic patients. CARD14 is mainly expressed in epidermal keratinocytes. TLR4 is a transmembrane protein to recognize microbial antigens. Our study aimed to assess the relationship among PSORS1C3, CARD14 and TLR4 polymorphisms, inflammatory expression and psoriasis susceptibility. To the end, 71 patients with psoriasis and 46 healthy individuals with the well-characterized clinical profiles were enrolled. Gene polymorphisms were determined by Sanger DNA sequencing and secretion of cytokines by ELISA. As a result, genetic analysis of PSORS1C3 gene identified nine SNPs and three haplotype blocks. Sequencing of the CARD14 gene determined eight SNPs and one haplotype block. Sequencing of TLR4 gene identified nine SNPs, in which a SNP rs1018673641 was found to exert deleterious effect. The linkage disequilibrium analysis showed that seven variants in PSORS1C3 gene and three SNPs in CARD14 gene were in tightly linked. More importantly, a significant association between IL-6 level and rs1018673641 AT genotype in TLR4 gene was detected in psoriatic patients. In conclusion, the PSORS1C3, CARD14 and TLR4 polymorphisms and haplotypes may be correlated with risk of suffering psoriasis and the IL-6-mediated chronic inflammation in psoriasis could be partially regulated by the TLR4 functional variant. |
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Genetics and Molecular Biology |
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Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibilityCARD14polymorphismPSORS1C3psoriasisTLR4Abstract Psoriasis is a common chronic, immune-mediated inflammatory disease of the skin. PSORS1C3 is a non-protein coding gene, of which the RNA transcript is found in psoriatic patients. CARD14 is mainly expressed in epidermal keratinocytes. TLR4 is a transmembrane protein to recognize microbial antigens. Our study aimed to assess the relationship among PSORS1C3, CARD14 and TLR4 polymorphisms, inflammatory expression and psoriasis susceptibility. To the end, 71 patients with psoriasis and 46 healthy individuals with the well-characterized clinical profiles were enrolled. Gene polymorphisms were determined by Sanger DNA sequencing and secretion of cytokines by ELISA. As a result, genetic analysis of PSORS1C3 gene identified nine SNPs and three haplotype blocks. Sequencing of the CARD14 gene determined eight SNPs and one haplotype block. Sequencing of TLR4 gene identified nine SNPs, in which a SNP rs1018673641 was found to exert deleterious effect. The linkage disequilibrium analysis showed that seven variants in PSORS1C3 gene and three SNPs in CARD14 gene were in tightly linked. More importantly, a significant association between IL-6 level and rs1018673641 AT genotype in TLR4 gene was detected in psoriatic patients. In conclusion, the PSORS1C3, CARD14 and TLR4 polymorphisms and haplotypes may be correlated with risk of suffering psoriasis and the IL-6-mediated chronic inflammation in psoriasis could be partially regulated by the TLR4 functional variant.Sociedade Brasileira de Genética2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500103Genetics and Molecular Biology v.45 n.4 2022reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2022-0099info:eu-repo/semantics/openAccessLinh,Nguyen Thi ThuyGiang,Nguyen HoangLien,Nguyen Thi KimTrang,Bui KieuTrang,Do ThiNgoc,Nguyen ThyNghia,Vu XuanMy,Le TraMao,Can VanHoang,Nguyen HuyXuan,Nguyen Thieng2022-11-03T00:00:00Zoai:scielo:S1415-47572022000500103Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2022-11-03T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility |
title |
Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility |
spellingShingle |
Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility Linh,Nguyen Thi Thuy CARD14 polymorphism PSORS1C3 psoriasis TLR4 |
title_short |
Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility |
title_full |
Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility |
title_fullStr |
Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility |
title_full_unstemmed |
Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility |
title_sort |
Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility |
author |
Linh,Nguyen Thi Thuy |
author_facet |
Linh,Nguyen Thi Thuy Giang,Nguyen Hoang Lien,Nguyen Thi Kim Trang,Bui Kieu Trang,Do Thi Ngoc,Nguyen Thy Nghia,Vu Xuan My,Le Tra Mao,Can Van Hoang,Nguyen Huy Xuan,Nguyen Thi |
author_role |
author |
author2 |
Giang,Nguyen Hoang Lien,Nguyen Thi Kim Trang,Bui Kieu Trang,Do Thi Ngoc,Nguyen Thy Nghia,Vu Xuan My,Le Tra Mao,Can Van Hoang,Nguyen Huy Xuan,Nguyen Thi |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Linh,Nguyen Thi Thuy Giang,Nguyen Hoang Lien,Nguyen Thi Kim Trang,Bui Kieu Trang,Do Thi Ngoc,Nguyen Thy Nghia,Vu Xuan My,Le Tra Mao,Can Van Hoang,Nguyen Huy Xuan,Nguyen Thi |
dc.subject.por.fl_str_mv |
CARD14 polymorphism PSORS1C3 psoriasis TLR4 |
topic |
CARD14 polymorphism PSORS1C3 psoriasis TLR4 |
description |
Abstract Psoriasis is a common chronic, immune-mediated inflammatory disease of the skin. PSORS1C3 is a non-protein coding gene, of which the RNA transcript is found in psoriatic patients. CARD14 is mainly expressed in epidermal keratinocytes. TLR4 is a transmembrane protein to recognize microbial antigens. Our study aimed to assess the relationship among PSORS1C3, CARD14 and TLR4 polymorphisms, inflammatory expression and psoriasis susceptibility. To the end, 71 patients with psoriasis and 46 healthy individuals with the well-characterized clinical profiles were enrolled. Gene polymorphisms were determined by Sanger DNA sequencing and secretion of cytokines by ELISA. As a result, genetic analysis of PSORS1C3 gene identified nine SNPs and three haplotype blocks. Sequencing of the CARD14 gene determined eight SNPs and one haplotype block. Sequencing of TLR4 gene identified nine SNPs, in which a SNP rs1018673641 was found to exert deleterious effect. The linkage disequilibrium analysis showed that seven variants in PSORS1C3 gene and three SNPs in CARD14 gene were in tightly linked. More importantly, a significant association between IL-6 level and rs1018673641 AT genotype in TLR4 gene was detected in psoriatic patients. In conclusion, the PSORS1C3, CARD14 and TLR4 polymorphisms and haplotypes may be correlated with risk of suffering psoriasis and the IL-6-mediated chronic inflammation in psoriasis could be partially regulated by the TLR4 functional variant. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500103 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500103 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2022-0099 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.45 n.4 2022 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122390708486144 |