Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility

Detalhes bibliográficos
Autor(a) principal: Linh,Nguyen Thi Thuy
Data de Publicação: 2022
Outros Autores: Giang,Nguyen Hoang, Lien,Nguyen Thi Kim, Trang,Bui Kieu, Trang,Do Thi, Ngoc,Nguyen Thy, Nghia,Vu Xuan, My,Le Tra, Mao,Can Van, Hoang,Nguyen Huy, Xuan,Nguyen Thi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500103
Resumo: Abstract Psoriasis is a common chronic, immune-mediated inflammatory disease of the skin. PSORS1C3 is a non-protein coding gene, of which the RNA transcript is found in psoriatic patients. CARD14 is mainly expressed in epidermal keratinocytes. TLR4 is a transmembrane protein to recognize microbial antigens. Our study aimed to assess the relationship among PSORS1C3, CARD14 and TLR4 polymorphisms, inflammatory expression and psoriasis susceptibility. To the end, 71 patients with psoriasis and 46 healthy individuals with the well-characterized clinical profiles were enrolled. Gene polymorphisms were determined by Sanger DNA sequencing and secretion of cytokines by ELISA. As a result, genetic analysis of PSORS1C3 gene identified nine SNPs and three haplotype blocks. Sequencing of the CARD14 gene determined eight SNPs and one haplotype block. Sequencing of TLR4 gene identified nine SNPs, in which a SNP rs1018673641 was found to exert deleterious effect. The linkage disequilibrium analysis showed that seven variants in PSORS1C3 gene and three SNPs in CARD14 gene were in tightly linked. More importantly, a significant association between IL-6 level and rs1018673641 AT genotype in TLR4 gene was detected in psoriatic patients. In conclusion, the PSORS1C3, CARD14 and TLR4 polymorphisms and haplotypes may be correlated with risk of suffering psoriasis and the IL-6-mediated chronic inflammation in psoriasis could be partially regulated by the TLR4 functional variant.
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spelling Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibilityCARD14polymorphismPSORS1C3psoriasisTLR4Abstract Psoriasis is a common chronic, immune-mediated inflammatory disease of the skin. PSORS1C3 is a non-protein coding gene, of which the RNA transcript is found in psoriatic patients. CARD14 is mainly expressed in epidermal keratinocytes. TLR4 is a transmembrane protein to recognize microbial antigens. Our study aimed to assess the relationship among PSORS1C3, CARD14 and TLR4 polymorphisms, inflammatory expression and psoriasis susceptibility. To the end, 71 patients with psoriasis and 46 healthy individuals with the well-characterized clinical profiles were enrolled. Gene polymorphisms were determined by Sanger DNA sequencing and secretion of cytokines by ELISA. As a result, genetic analysis of PSORS1C3 gene identified nine SNPs and three haplotype blocks. Sequencing of the CARD14 gene determined eight SNPs and one haplotype block. Sequencing of TLR4 gene identified nine SNPs, in which a SNP rs1018673641 was found to exert deleterious effect. The linkage disequilibrium analysis showed that seven variants in PSORS1C3 gene and three SNPs in CARD14 gene were in tightly linked. More importantly, a significant association between IL-6 level and rs1018673641 AT genotype in TLR4 gene was detected in psoriatic patients. In conclusion, the PSORS1C3, CARD14 and TLR4 polymorphisms and haplotypes may be correlated with risk of suffering psoriasis and the IL-6-mediated chronic inflammation in psoriasis could be partially regulated by the TLR4 functional variant.Sociedade Brasileira de Genética2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500103Genetics and Molecular Biology v.45 n.4 2022reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2022-0099info:eu-repo/semantics/openAccessLinh,Nguyen Thi ThuyGiang,Nguyen HoangLien,Nguyen Thi KimTrang,Bui KieuTrang,Do ThiNgoc,Nguyen ThyNghia,Vu XuanMy,Le TraMao,Can VanHoang,Nguyen HuyXuan,Nguyen Thieng2022-11-03T00:00:00Zoai:scielo:S1415-47572022000500103Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2022-11-03T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility
title Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility
spellingShingle Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility
Linh,Nguyen Thi Thuy
CARD14
polymorphism
PSORS1C3
psoriasis
TLR4
title_short Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility
title_full Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility
title_fullStr Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility
title_full_unstemmed Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility
title_sort Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility
author Linh,Nguyen Thi Thuy
author_facet Linh,Nguyen Thi Thuy
Giang,Nguyen Hoang
Lien,Nguyen Thi Kim
Trang,Bui Kieu
Trang,Do Thi
Ngoc,Nguyen Thy
Nghia,Vu Xuan
My,Le Tra
Mao,Can Van
Hoang,Nguyen Huy
Xuan,Nguyen Thi
author_role author
author2 Giang,Nguyen Hoang
Lien,Nguyen Thi Kim
Trang,Bui Kieu
Trang,Do Thi
Ngoc,Nguyen Thy
Nghia,Vu Xuan
My,Le Tra
Mao,Can Van
Hoang,Nguyen Huy
Xuan,Nguyen Thi
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Linh,Nguyen Thi Thuy
Giang,Nguyen Hoang
Lien,Nguyen Thi Kim
Trang,Bui Kieu
Trang,Do Thi
Ngoc,Nguyen Thy
Nghia,Vu Xuan
My,Le Tra
Mao,Can Van
Hoang,Nguyen Huy
Xuan,Nguyen Thi
dc.subject.por.fl_str_mv CARD14
polymorphism
PSORS1C3
psoriasis
TLR4
topic CARD14
polymorphism
PSORS1C3
psoriasis
TLR4
description Abstract Psoriasis is a common chronic, immune-mediated inflammatory disease of the skin. PSORS1C3 is a non-protein coding gene, of which the RNA transcript is found in psoriatic patients. CARD14 is mainly expressed in epidermal keratinocytes. TLR4 is a transmembrane protein to recognize microbial antigens. Our study aimed to assess the relationship among PSORS1C3, CARD14 and TLR4 polymorphisms, inflammatory expression and psoriasis susceptibility. To the end, 71 patients with psoriasis and 46 healthy individuals with the well-characterized clinical profiles were enrolled. Gene polymorphisms were determined by Sanger DNA sequencing and secretion of cytokines by ELISA. As a result, genetic analysis of PSORS1C3 gene identified nine SNPs and three haplotype blocks. Sequencing of the CARD14 gene determined eight SNPs and one haplotype block. Sequencing of TLR4 gene identified nine SNPs, in which a SNP rs1018673641 was found to exert deleterious effect. The linkage disequilibrium analysis showed that seven variants in PSORS1C3 gene and three SNPs in CARD14 gene were in tightly linked. More importantly, a significant association between IL-6 level and rs1018673641 AT genotype in TLR4 gene was detected in psoriatic patients. In conclusion, the PSORS1C3, CARD14 and TLR4 polymorphisms and haplotypes may be correlated with risk of suffering psoriasis and the IL-6-mediated chronic inflammation in psoriasis could be partially regulated by the TLR4 functional variant.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500103
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500103
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2022-0099
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.45 n.4 2022
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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