Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C

Detalhes bibliográficos
Autor(a) principal: Jiao,Yan
Data de Publicação: 2011
Outros Autores: Zhang,Jifei, Yan,Jian, Stuart,John, Gibson,Griffin, Lu,Lu, Willaims,Robert, Wang,Yong Jun, Gu,Weikuan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300004
Resumo: The aim of this study was to test the hypothesis that hepatic vitamin C (VC) levels in VC deficient mice rescued with high doses of VC supplements still do not reach the optimal levels present in wild-type mice. For this, we used a mouse scurvy model (sfx) in which the L-gulonolactone oxidase gene (Gulo) is deleted. Six age- (6 weeks old) and gender- (female) matched wild-type (WT) and sfx mice (rescued by administering 500 mg of VC/L) were used as the control (WT) and treatment (MT) groups (n = 3 for each group), respectively. Total hepatic RNA was used in triplicate microarray assays for each group. EDGE software was used to identify differentially expressed genes and transcriptomic analysis was used to assess the potential genetic regulation of Gulo gene expression. Hepatic VC concentrations in MT mice were significantly lower than in WT mice, even though there were no morphological differences between the two groups. In MT mice, 269 differentially expressed transcripts were detected (> twice the difference between MT and WT mice), including 107 up-regulated and 162 down-regulated genes. These differentially expressed genes included stress-related and exclusively/predominantly hepatocyte genes. Transcriptomic analysis identified a major locus on chromosome 18 that regulates Gulo expression. Since three relevant oxidative genes are located within the critical region of this locus we suspect that they are involved in the down-regulation of oxidative activity in sfx mice.
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spelling Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin Cgene expressionL-gulonolactone oxidaseliveroxidative stressvitamin CThe aim of this study was to test the hypothesis that hepatic vitamin C (VC) levels in VC deficient mice rescued with high doses of VC supplements still do not reach the optimal levels present in wild-type mice. For this, we used a mouse scurvy model (sfx) in which the L-gulonolactone oxidase gene (Gulo) is deleted. Six age- (6 weeks old) and gender- (female) matched wild-type (WT) and sfx mice (rescued by administering 500 mg of VC/L) were used as the control (WT) and treatment (MT) groups (n = 3 for each group), respectively. Total hepatic RNA was used in triplicate microarray assays for each group. EDGE software was used to identify differentially expressed genes and transcriptomic analysis was used to assess the potential genetic regulation of Gulo gene expression. Hepatic VC concentrations in MT mice were significantly lower than in WT mice, even though there were no morphological differences between the two groups. In MT mice, 269 differentially expressed transcripts were detected (> twice the difference between MT and WT mice), including 107 up-regulated and 162 down-regulated genes. These differentially expressed genes included stress-related and exclusively/predominantly hepatocyte genes. Transcriptomic analysis identified a major locus on chromosome 18 that regulates Gulo expression. Since three relevant oxidative genes are located within the critical region of this locus we suspect that they are involved in the down-regulation of oxidative activity in sfx mice.Sociedade Brasileira de Genética2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300004Genetics and Molecular Biology v.34 n.3 2011reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572011005000031info:eu-repo/semantics/openAccessJiao,YanZhang,JifeiYan,JianStuart,JohnGibson,GriffinLu,LuWillaims,RobertWang,Yong JunGu,Weikuaneng2012-03-09T00:00:00Zoai:scielo:S1415-47572011000300004Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2012-03-09T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
title Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
spellingShingle Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
Jiao,Yan
gene expression
L-gulonolactone oxidase
liver
oxidative stress
vitamin C
title_short Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
title_full Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
title_fullStr Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
title_full_unstemmed Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
title_sort Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
author Jiao,Yan
author_facet Jiao,Yan
Zhang,Jifei
Yan,Jian
Stuart,John
Gibson,Griffin
Lu,Lu
Willaims,Robert
Wang,Yong Jun
Gu,Weikuan
author_role author
author2 Zhang,Jifei
Yan,Jian
Stuart,John
Gibson,Griffin
Lu,Lu
Willaims,Robert
Wang,Yong Jun
Gu,Weikuan
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jiao,Yan
Zhang,Jifei
Yan,Jian
Stuart,John
Gibson,Griffin
Lu,Lu
Willaims,Robert
Wang,Yong Jun
Gu,Weikuan
dc.subject.por.fl_str_mv gene expression
L-gulonolactone oxidase
liver
oxidative stress
vitamin C
topic gene expression
L-gulonolactone oxidase
liver
oxidative stress
vitamin C
description The aim of this study was to test the hypothesis that hepatic vitamin C (VC) levels in VC deficient mice rescued with high doses of VC supplements still do not reach the optimal levels present in wild-type mice. For this, we used a mouse scurvy model (sfx) in which the L-gulonolactone oxidase gene (Gulo) is deleted. Six age- (6 weeks old) and gender- (female) matched wild-type (WT) and sfx mice (rescued by administering 500 mg of VC/L) were used as the control (WT) and treatment (MT) groups (n = 3 for each group), respectively. Total hepatic RNA was used in triplicate microarray assays for each group. EDGE software was used to identify differentially expressed genes and transcriptomic analysis was used to assess the potential genetic regulation of Gulo gene expression. Hepatic VC concentrations in MT mice were significantly lower than in WT mice, even though there were no morphological differences between the two groups. In MT mice, 269 differentially expressed transcripts were detected (> twice the difference between MT and WT mice), including 107 up-regulated and 162 down-regulated genes. These differentially expressed genes included stress-related and exclusively/predominantly hepatocyte genes. Transcriptomic analysis identified a major locus on chromosome 18 that regulates Gulo expression. Since three relevant oxidative genes are located within the critical region of this locus we suspect that they are involved in the down-regulation of oxidative activity in sfx mice.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572011005000031
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.34 n.3 2011
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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