Characterization of 13 microsatellite loci developed from Meconopsis horridula

Detalhes bibliográficos
Autor(a) principal: Zhao,Yu
Data de Publicação: 2010
Outros Autores: Zhang,Shi-Bao, Yang,Jing, Zhang,Lu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300026
Resumo: Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs.
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spelling Characterization of 13 microsatellite loci developed from Meconopsis horridulagenetic structureheterozygosityMeconopsis horridulamicrosatellite markerspolymorphismMeconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs.Sociedade Brasileira de Genética2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300026Genetics and Molecular Biology v.33 n.3 2010reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572010005000066info:eu-repo/semantics/openAccessZhao,YuZhang,Shi-BaoYang,JingZhang,Lueng2010-08-18T00:00:00Zoai:scielo:S1415-47572010000300026Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2010-08-18T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Characterization of 13 microsatellite loci developed from Meconopsis horridula
title Characterization of 13 microsatellite loci developed from Meconopsis horridula
spellingShingle Characterization of 13 microsatellite loci developed from Meconopsis horridula
Zhao,Yu
genetic structure
heterozygosity
Meconopsis horridula
microsatellite markers
polymorphism
title_short Characterization of 13 microsatellite loci developed from Meconopsis horridula
title_full Characterization of 13 microsatellite loci developed from Meconopsis horridula
title_fullStr Characterization of 13 microsatellite loci developed from Meconopsis horridula
title_full_unstemmed Characterization of 13 microsatellite loci developed from Meconopsis horridula
title_sort Characterization of 13 microsatellite loci developed from Meconopsis horridula
author Zhao,Yu
author_facet Zhao,Yu
Zhang,Shi-Bao
Yang,Jing
Zhang,Lu
author_role author
author2 Zhang,Shi-Bao
Yang,Jing
Zhang,Lu
author2_role author
author
author
dc.contributor.author.fl_str_mv Zhao,Yu
Zhang,Shi-Bao
Yang,Jing
Zhang,Lu
dc.subject.por.fl_str_mv genetic structure
heterozygosity
Meconopsis horridula
microsatellite markers
polymorphism
topic genetic structure
heterozygosity
Meconopsis horridula
microsatellite markers
polymorphism
description Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300026
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300026
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572010005000066
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.33 n.3 2010
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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