Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair

Detalhes bibliográficos
Autor(a) principal: Wits,Maria Inês
Data de Publicação: 2020
Outros Autores: Tobin,Gabriela Cabanas, Silveira,Maiele Dornelles, Baja,Karine Gehlen, Braga,Luisa Maria Macedo, Sesterheim,Patricia, Camassola,Melissa, Nardi,Nance Beyer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701
Resumo: Abstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical, orthopedic and radiological parameters. Lameness at walk and pain on manipulation were reduced in the ASC group and mainly in the ASC/HA group. Range of motion and detection of crepitus on hip rotation and abduction improved similarly in all groups. For articular edema, muscle atrophy, Norberg angle values and radiographic analyses, there were no variations throughout the period. These results indicate that ASC/HA constructs are safe and may be an effective therapeutic tool in treating canine chronic osteoarthritis, which should be confirmed with larger studies and additional clinical parameters.
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spelling Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repairMesenchymal stromal cellshyaluronic acidosteoarthritisdogAbstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical, orthopedic and radiological parameters. Lameness at walk and pain on manipulation were reduced in the ASC group and mainly in the ASC/HA group. Range of motion and detection of crepitus on hip rotation and abduction improved similarly in all groups. For articular edema, muscle atrophy, Norberg angle values and radiographic analyses, there were no variations throughout the period. These results indicate that ASC/HA constructs are safe and may be an effective therapeutic tool in treating canine chronic osteoarthritis, which should be confirmed with larger studies and additional clinical parameters.Sociedade Brasileira de Genética2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701Genetics and Molecular Biology v.43 n.1 2020reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2019-0275info:eu-repo/semantics/openAccessWits,Maria InêsTobin,Gabriela CabanasSilveira,Maiele DornellesBaja,Karine GehlenBraga,Luisa Maria MacedoSesterheim,PatriciaCamassola,MelissaNardi,Nance Beyereng2020-02-28T00:00:00Zoai:scielo:S1415-47572020000100701Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2020-02-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
title Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
spellingShingle Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
Wits,Maria Inês
Mesenchymal stromal cells
hyaluronic acid
osteoarthritis
dog
title_short Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
title_full Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
title_fullStr Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
title_full_unstemmed Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
title_sort Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
author Wits,Maria Inês
author_facet Wits,Maria Inês
Tobin,Gabriela Cabanas
Silveira,Maiele Dornelles
Baja,Karine Gehlen
Braga,Luisa Maria Macedo
Sesterheim,Patricia
Camassola,Melissa
Nardi,Nance Beyer
author_role author
author2 Tobin,Gabriela Cabanas
Silveira,Maiele Dornelles
Baja,Karine Gehlen
Braga,Luisa Maria Macedo
Sesterheim,Patricia
Camassola,Melissa
Nardi,Nance Beyer
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wits,Maria Inês
Tobin,Gabriela Cabanas
Silveira,Maiele Dornelles
Baja,Karine Gehlen
Braga,Luisa Maria Macedo
Sesterheim,Patricia
Camassola,Melissa
Nardi,Nance Beyer
dc.subject.por.fl_str_mv Mesenchymal stromal cells
hyaluronic acid
osteoarthritis
dog
topic Mesenchymal stromal cells
hyaluronic acid
osteoarthritis
dog
description Abstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical, orthopedic and radiological parameters. Lameness at walk and pain on manipulation were reduced in the ASC group and mainly in the ASC/HA group. Range of motion and detection of crepitus on hip rotation and abduction improved similarly in all groups. For articular edema, muscle atrophy, Norberg angle values and radiographic analyses, there were no variations throughout the period. These results indicate that ASC/HA constructs are safe and may be an effective therapeutic tool in treating canine chronic osteoarthritis, which should be confirmed with larger studies and additional clinical parameters.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2019-0275
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.43 n.1 2020
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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