Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701 |
Resumo: | Abstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical, orthopedic and radiological parameters. Lameness at walk and pain on manipulation were reduced in the ASC group and mainly in the ASC/HA group. Range of motion and detection of crepitus on hip rotation and abduction improved similarly in all groups. For articular edema, muscle atrophy, Norberg angle values and radiographic analyses, there were no variations throughout the period. These results indicate that ASC/HA constructs are safe and may be an effective therapeutic tool in treating canine chronic osteoarthritis, which should be confirmed with larger studies and additional clinical parameters. |
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Genetics and Molecular Biology |
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Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repairMesenchymal stromal cellshyaluronic acidosteoarthritisdogAbstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical, orthopedic and radiological parameters. Lameness at walk and pain on manipulation were reduced in the ASC group and mainly in the ASC/HA group. Range of motion and detection of crepitus on hip rotation and abduction improved similarly in all groups. For articular edema, muscle atrophy, Norberg angle values and radiographic analyses, there were no variations throughout the period. These results indicate that ASC/HA constructs are safe and may be an effective therapeutic tool in treating canine chronic osteoarthritis, which should be confirmed with larger studies and additional clinical parameters.Sociedade Brasileira de Genética2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701Genetics and Molecular Biology v.43 n.1 2020reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2019-0275info:eu-repo/semantics/openAccessWits,Maria InêsTobin,Gabriela CabanasSilveira,Maiele DornellesBaja,Karine GehlenBraga,Luisa Maria MacedoSesterheim,PatriciaCamassola,MelissaNardi,Nance Beyereng2020-02-28T00:00:00Zoai:scielo:S1415-47572020000100701Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2020-02-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair |
title |
Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair |
spellingShingle |
Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair Wits,Maria Inês Mesenchymal stromal cells hyaluronic acid osteoarthritis dog |
title_short |
Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair |
title_full |
Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair |
title_fullStr |
Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair |
title_full_unstemmed |
Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair |
title_sort |
Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair |
author |
Wits,Maria Inês |
author_facet |
Wits,Maria Inês Tobin,Gabriela Cabanas Silveira,Maiele Dornelles Baja,Karine Gehlen Braga,Luisa Maria Macedo Sesterheim,Patricia Camassola,Melissa Nardi,Nance Beyer |
author_role |
author |
author2 |
Tobin,Gabriela Cabanas Silveira,Maiele Dornelles Baja,Karine Gehlen Braga,Luisa Maria Macedo Sesterheim,Patricia Camassola,Melissa Nardi,Nance Beyer |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Wits,Maria Inês Tobin,Gabriela Cabanas Silveira,Maiele Dornelles Baja,Karine Gehlen Braga,Luisa Maria Macedo Sesterheim,Patricia Camassola,Melissa Nardi,Nance Beyer |
dc.subject.por.fl_str_mv |
Mesenchymal stromal cells hyaluronic acid osteoarthritis dog |
topic |
Mesenchymal stromal cells hyaluronic acid osteoarthritis dog |
description |
Abstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical, orthopedic and radiological parameters. Lameness at walk and pain on manipulation were reduced in the ASC group and mainly in the ASC/HA group. Range of motion and detection of crepitus on hip rotation and abduction improved similarly in all groups. For articular edema, muscle atrophy, Norberg angle values and radiographic analyses, there were no variations throughout the period. These results indicate that ASC/HA constructs are safe and may be an effective therapeutic tool in treating canine chronic osteoarthritis, which should be confirmed with larger studies and additional clinical parameters. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2019-0275 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.43 n.1 2020 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122389698707456 |