Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)

Detalhes bibliográficos
Autor(a) principal: Aquino-Silva,Maria Regina de
Data de Publicação: 1998
Outros Autores: Schwantes,Maria Luiza B., Schwantes,Arno Rudi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400016
Resumo: A recent locus duplication hypothesis for sMDH-B* was proposed to explain the complex electrophoretic pattern of six bands detected for the soluble form of malate dehydrogenase (MDH, EC 1.1.1.37) in 84% of the Geophagus brasiliensis (Cichlidae, Perciformes) analyzed (AB1B2 individuals). Klebe's serial dilutions were carried out in skeletal muscle extracts. B1 and B2 subunits had the same visual end-points, reflecting a nondivergent pattern for these B-duplicated genes. Since there is no evidence of polyploidy in the Cichlidae family, MDH-B* loci must have evolved from regional gene duplication. Tissue specificities, thermostability and kinetic tests resulted in similar responses from both B-isoforms, in both sMDH phenotypes, suggesting that these more recently duplicated loci underwent the same regulatory gene action. Similar results obtained with the two sMDH phenotypes did not show any indication of a six-banded specimen adaptive advantage in subtropical regions.
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spelling Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)A recent locus duplication hypothesis for sMDH-B* was proposed to explain the complex electrophoretic pattern of six bands detected for the soluble form of malate dehydrogenase (MDH, EC 1.1.1.37) in 84% of the Geophagus brasiliensis (Cichlidae, Perciformes) analyzed (AB1B2 individuals). Klebe's serial dilutions were carried out in skeletal muscle extracts. B1 and B2 subunits had the same visual end-points, reflecting a nondivergent pattern for these B-duplicated genes. Since there is no evidence of polyploidy in the Cichlidae family, MDH-B* loci must have evolved from regional gene duplication. Tissue specificities, thermostability and kinetic tests resulted in similar responses from both B-isoforms, in both sMDH phenotypes, suggesting that these more recently duplicated loci underwent the same regulatory gene action. Similar results obtained with the two sMDH phenotypes did not show any indication of a six-banded specimen adaptive advantage in subtropical regions.Sociedade Brasileira de Genética1998-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400016Genetics and Molecular Biology v.21 n.4 1998reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571998000400016info:eu-repo/semantics/openAccessAquino-Silva,Maria Regina deSchwantes,Maria Luiza B.Schwantes,Arno Rudieng1999-03-01T00:00:00Zoai:scielo:S1415-47571998000400016Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-03-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
title Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
spellingShingle Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
Aquino-Silva,Maria Regina de
title_short Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
title_full Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
title_fullStr Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
title_full_unstemmed Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
title_sort Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
author Aquino-Silva,Maria Regina de
author_facet Aquino-Silva,Maria Regina de
Schwantes,Maria Luiza B.
Schwantes,Arno Rudi
author_role author
author2 Schwantes,Maria Luiza B.
Schwantes,Arno Rudi
author2_role author
author
dc.contributor.author.fl_str_mv Aquino-Silva,Maria Regina de
Schwantes,Maria Luiza B.
Schwantes,Arno Rudi
description A recent locus duplication hypothesis for sMDH-B* was proposed to explain the complex electrophoretic pattern of six bands detected for the soluble form of malate dehydrogenase (MDH, EC 1.1.1.37) in 84% of the Geophagus brasiliensis (Cichlidae, Perciformes) analyzed (AB1B2 individuals). Klebe's serial dilutions were carried out in skeletal muscle extracts. B1 and B2 subunits had the same visual end-points, reflecting a nondivergent pattern for these B-duplicated genes. Since there is no evidence of polyploidy in the Cichlidae family, MDH-B* loci must have evolved from regional gene duplication. Tissue specificities, thermostability and kinetic tests resulted in similar responses from both B-isoforms, in both sMDH phenotypes, suggesting that these more recently duplicated loci underwent the same regulatory gene action. Similar results obtained with the two sMDH phenotypes did not show any indication of a six-banded specimen adaptive advantage in subtropical regions.
publishDate 1998
dc.date.none.fl_str_mv 1998-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400016
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47571998000400016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.21 n.4 1998
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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