Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)

Detalhes bibliográficos
Autor(a) principal: Tavares,Mara Garcia
Data de Publicação: 1998
Outros Autores: Azeredo-Oliveira,Maria Tercilia Vilela de, Ceron,Carlos Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400009
Resumo: We examined the esterases present in the hemolymph and Malpighian tubules of "Kissing bug", Triatoma infestans (Triatominae, Heteroptera) by polyacrylamide gel electrophoresis. Six esterase bands were observed and were designated EST 1 to EST 6. EST 1, 4, 5 and 6 were exclusive to hemolymph, whereas EST 2 and 3 were found only in Malpighian tubules. Each tissue had a characteristic esterase pattern, which may be related to its functional role. The four hemolymph esterases hydrolyzed a-naphthyl acetate. One of these enzymes was classified as a carboxylesterase (EST 4), and another was an acetylesterase (EST 6). The other two enzymes (EST 1 and 5) could be either carboxylesterases or serino-proteases with an esterolytic function, as they were selectively inhibited by phenylmethylsulfonyl fluoride (PMSF). Absence of genetic variability could be due to high inbreeding.
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spelling Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)We examined the esterases present in the hemolymph and Malpighian tubules of "Kissing bug", Triatoma infestans (Triatominae, Heteroptera) by polyacrylamide gel electrophoresis. Six esterase bands were observed and were designated EST 1 to EST 6. EST 1, 4, 5 and 6 were exclusive to hemolymph, whereas EST 2 and 3 were found only in Malpighian tubules. Each tissue had a characteristic esterase pattern, which may be related to its functional role. The four hemolymph esterases hydrolyzed a-naphthyl acetate. One of these enzymes was classified as a carboxylesterase (EST 4), and another was an acetylesterase (EST 6). The other two enzymes (EST 1 and 5) could be either carboxylesterases or serino-proteases with an esterolytic function, as they were selectively inhibited by phenylmethylsulfonyl fluoride (PMSF). Absence of genetic variability could be due to high inbreeding.Sociedade Brasileira de Genética1998-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400009Genetics and Molecular Biology v.21 n.4 1998reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571998000400009info:eu-repo/semantics/openAccessTavares,Mara GarciaAzeredo-Oliveira,Maria Tercilia Vilela deCeron,Carlos Robertoeng1999-03-01T00:00:00Zoai:scielo:S1415-47571998000400009Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-03-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
title Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
spellingShingle Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
Tavares,Mara Garcia
title_short Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
title_full Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
title_fullStr Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
title_full_unstemmed Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
title_sort Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
author Tavares,Mara Garcia
author_facet Tavares,Mara Garcia
Azeredo-Oliveira,Maria Tercilia Vilela de
Ceron,Carlos Roberto
author_role author
author2 Azeredo-Oliveira,Maria Tercilia Vilela de
Ceron,Carlos Roberto
author2_role author
author
dc.contributor.author.fl_str_mv Tavares,Mara Garcia
Azeredo-Oliveira,Maria Tercilia Vilela de
Ceron,Carlos Roberto
description We examined the esterases present in the hemolymph and Malpighian tubules of "Kissing bug", Triatoma infestans (Triatominae, Heteroptera) by polyacrylamide gel electrophoresis. Six esterase bands were observed and were designated EST 1 to EST 6. EST 1, 4, 5 and 6 were exclusive to hemolymph, whereas EST 2 and 3 were found only in Malpighian tubules. Each tissue had a characteristic esterase pattern, which may be related to its functional role. The four hemolymph esterases hydrolyzed a-naphthyl acetate. One of these enzymes was classified as a carboxylesterase (EST 4), and another was an acetylesterase (EST 6). The other two enzymes (EST 1 and 5) could be either carboxylesterases or serino-proteases with an esterolytic function, as they were selectively inhibited by phenylmethylsulfonyl fluoride (PMSF). Absence of genetic variability could be due to high inbreeding.
publishDate 1998
dc.date.none.fl_str_mv 1998-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47571998000400009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.21 n.4 1998
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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