DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses

Detalhes bibliográficos
Autor(a) principal: Pinto,S.M.
Data de Publicação: 2000
Outros Autores: Fernandes-Matioli,F.M.C., Schlenz,E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300017
Resumo: A specific DNA extraction method for sea anemones is described in which extraction of total DNA from eight species of sea anemones and one species of corallimorpharian was achieved by changing the standard extraction protocols. DNA extraction from sea anemone tissue is made more difficult both by the tissue consistency and the presence of symbiotic zooxanthellae. The technique described here is an efficient way to avoid problems of DNA contamination and obtain large amounts of purified and integral DNA which can be used in different kinds of molecular analyses.
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spelling DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analysesA specific DNA extraction method for sea anemones is described in which extraction of total DNA from eight species of sea anemones and one species of corallimorpharian was achieved by changing the standard extraction protocols. DNA extraction from sea anemone tissue is made more difficult both by the tissue consistency and the presence of symbiotic zooxanthellae. The technique described here is an efficient way to avoid problems of DNA contamination and obtain large amounts of purified and integral DNA which can be used in different kinds of molecular analyses.Sociedade Brasileira de Genética2000-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300017Genetics and Molecular Biology v.23 n.3 2000reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572000000300017info:eu-repo/semantics/openAccessPinto,S.M.Fernandes-Matioli,F.M.C.Schlenz,E.eng2001-05-04T00:00:00Zoai:scielo:S1415-47572000000300017Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2001-05-04T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses
title DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses
spellingShingle DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses
Pinto,S.M.
title_short DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses
title_full DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses
title_fullStr DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses
title_full_unstemmed DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses
title_sort DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses
author Pinto,S.M.
author_facet Pinto,S.M.
Fernandes-Matioli,F.M.C.
Schlenz,E.
author_role author
author2 Fernandes-Matioli,F.M.C.
Schlenz,E.
author2_role author
author
dc.contributor.author.fl_str_mv Pinto,S.M.
Fernandes-Matioli,F.M.C.
Schlenz,E.
description A specific DNA extraction method for sea anemones is described in which extraction of total DNA from eight species of sea anemones and one species of corallimorpharian was achieved by changing the standard extraction protocols. DNA extraction from sea anemone tissue is made more difficult both by the tissue consistency and the presence of symbiotic zooxanthellae. The technique described here is an efficient way to avoid problems of DNA contamination and obtain large amounts of purified and integral DNA which can be used in different kinds of molecular analyses.
publishDate 2000
dc.date.none.fl_str_mv 2000-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572000000300017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.23 n.3 2000
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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