Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients

Detalhes bibliográficos
Autor(a) principal: Del Valle,Paulo Roberto
Data de Publicação: 2014
Outros Autores: Milani,Cintia, Brentani,Maria Mitzi, Katayama,Maria Lucia Hirata, Lyra,Eduardo Carneiro de, Carraro,Dirce Maria, Brentani,Helena, Puga,Renato, Lima,Leandro A., Rozenchan,Patricia Bortman, Nunes,Bárbara dos Santos, Góes,João Carlos Guedes Sampaio, Folgueira,Maria Aparecida Azevedo Koike
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002
Resumo: Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were overrepresented. In a biological validation set, NOTCH2 was confirmed to be more expressed in N+ (vs CAF) and ADCY2, HECTD1, HNMT, LOX, MACF1, SLC1A3 and USP16 more expressed in BM (vs CAF). Only small differences were observed in the transcriptional profiles of fibroblasts from the primary tumor and lymph node of breast cancer patients, whereas greater differences were observed between bone marrow stromal cells and the other two sites. These differences may reflect the activities of distinct differentiation programs.
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spelling Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patientsbone marrowbreast neoplasmfibroblastgene expression profilinglymph nodemesenchymal stromal cellstumor microenvironmentCancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were overrepresented. In a biological validation set, NOTCH2 was confirmed to be more expressed in N+ (vs CAF) and ADCY2, HECTD1, HNMT, LOX, MACF1, SLC1A3 and USP16 more expressed in BM (vs CAF). Only small differences were observed in the transcriptional profiles of fibroblasts from the primary tumor and lymph node of breast cancer patients, whereas greater differences were observed between bone marrow stromal cells and the other two sites. These differences may reflect the activities of distinct differentiation programs.Sociedade Brasileira de Genética2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002Genetics and Molecular Biology v.37 n.3 2014reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572014000400002info:eu-repo/semantics/openAccessDel Valle,Paulo RobertoMilani,CintiaBrentani,Maria MitziKatayama,Maria Lucia HirataLyra,Eduardo Carneiro deCarraro,Dirce MariaBrentani,HelenaPuga,RenatoLima,Leandro A.Rozenchan,Patricia BortmanNunes,Bárbara dos SantosGóes,João Carlos Guedes SampaioFolgueira,Maria Aparecida Azevedo Koikeeng2014-09-22T00:00:00Zoai:scielo:S1415-47572014000400002Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2014-09-22T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
title Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
spellingShingle Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
Del Valle,Paulo Roberto
bone marrow
breast neoplasm
fibroblast
gene expression profiling
lymph node
mesenchymal stromal cells
tumor microenvironment
title_short Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
title_full Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
title_fullStr Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
title_full_unstemmed Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
title_sort Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
author Del Valle,Paulo Roberto
author_facet Del Valle,Paulo Roberto
Milani,Cintia
Brentani,Maria Mitzi
Katayama,Maria Lucia Hirata
Lyra,Eduardo Carneiro de
Carraro,Dirce Maria
Brentani,Helena
Puga,Renato
Lima,Leandro A.
Rozenchan,Patricia Bortman
Nunes,Bárbara dos Santos
Góes,João Carlos Guedes Sampaio
Folgueira,Maria Aparecida Azevedo Koike
author_role author
author2 Milani,Cintia
Brentani,Maria Mitzi
Katayama,Maria Lucia Hirata
Lyra,Eduardo Carneiro de
Carraro,Dirce Maria
Brentani,Helena
Puga,Renato
Lima,Leandro A.
Rozenchan,Patricia Bortman
Nunes,Bárbara dos Santos
Góes,João Carlos Guedes Sampaio
Folgueira,Maria Aparecida Azevedo Koike
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Del Valle,Paulo Roberto
Milani,Cintia
Brentani,Maria Mitzi
Katayama,Maria Lucia Hirata
Lyra,Eduardo Carneiro de
Carraro,Dirce Maria
Brentani,Helena
Puga,Renato
Lima,Leandro A.
Rozenchan,Patricia Bortman
Nunes,Bárbara dos Santos
Góes,João Carlos Guedes Sampaio
Folgueira,Maria Aparecida Azevedo Koike
dc.subject.por.fl_str_mv bone marrow
breast neoplasm
fibroblast
gene expression profiling
lymph node
mesenchymal stromal cells
tumor microenvironment
topic bone marrow
breast neoplasm
fibroblast
gene expression profiling
lymph node
mesenchymal stromal cells
tumor microenvironment
description Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were overrepresented. In a biological validation set, NOTCH2 was confirmed to be more expressed in N+ (vs CAF) and ADCY2, HECTD1, HNMT, LOX, MACF1, SLC1A3 and USP16 more expressed in BM (vs CAF). Only small differences were observed in the transcriptional profiles of fibroblasts from the primary tumor and lymph node of breast cancer patients, whereas greater differences were observed between bone marrow stromal cells and the other two sites. These differences may reflect the activities of distinct differentiation programs.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572014000400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.37 n.3 2014
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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