Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002 |
Resumo: | Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were overrepresented. In a biological validation set, NOTCH2 was confirmed to be more expressed in N+ (vs CAF) and ADCY2, HECTD1, HNMT, LOX, MACF1, SLC1A3 and USP16 more expressed in BM (vs CAF). Only small differences were observed in the transcriptional profiles of fibroblasts from the primary tumor and lymph node of breast cancer patients, whereas greater differences were observed between bone marrow stromal cells and the other two sites. These differences may reflect the activities of distinct differentiation programs. |
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Genetics and Molecular Biology |
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Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patientsbone marrowbreast neoplasmfibroblastgene expression profilinglymph nodemesenchymal stromal cellstumor microenvironmentCancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were overrepresented. In a biological validation set, NOTCH2 was confirmed to be more expressed in N+ (vs CAF) and ADCY2, HECTD1, HNMT, LOX, MACF1, SLC1A3 and USP16 more expressed in BM (vs CAF). Only small differences were observed in the transcriptional profiles of fibroblasts from the primary tumor and lymph node of breast cancer patients, whereas greater differences were observed between bone marrow stromal cells and the other two sites. These differences may reflect the activities of distinct differentiation programs.Sociedade Brasileira de Genética2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002Genetics and Molecular Biology v.37 n.3 2014reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572014000400002info:eu-repo/semantics/openAccessDel Valle,Paulo RobertoMilani,CintiaBrentani,Maria MitziKatayama,Maria Lucia HirataLyra,Eduardo Carneiro deCarraro,Dirce MariaBrentani,HelenaPuga,RenatoLima,Leandro A.Rozenchan,Patricia BortmanNunes,Bárbara dos SantosGóes,João Carlos Guedes SampaioFolgueira,Maria Aparecida Azevedo Koikeeng2014-09-22T00:00:00Zoai:scielo:S1415-47572014000400002Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2014-09-22T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title |
Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
spellingShingle |
Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients Del Valle,Paulo Roberto bone marrow breast neoplasm fibroblast gene expression profiling lymph node mesenchymal stromal cells tumor microenvironment |
title_short |
Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title_full |
Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title_fullStr |
Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title_full_unstemmed |
Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title_sort |
Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
author |
Del Valle,Paulo Roberto |
author_facet |
Del Valle,Paulo Roberto Milani,Cintia Brentani,Maria Mitzi Katayama,Maria Lucia Hirata Lyra,Eduardo Carneiro de Carraro,Dirce Maria Brentani,Helena Puga,Renato Lima,Leandro A. Rozenchan,Patricia Bortman Nunes,Bárbara dos Santos Góes,João Carlos Guedes Sampaio Folgueira,Maria Aparecida Azevedo Koike |
author_role |
author |
author2 |
Milani,Cintia Brentani,Maria Mitzi Katayama,Maria Lucia Hirata Lyra,Eduardo Carneiro de Carraro,Dirce Maria Brentani,Helena Puga,Renato Lima,Leandro A. Rozenchan,Patricia Bortman Nunes,Bárbara dos Santos Góes,João Carlos Guedes Sampaio Folgueira,Maria Aparecida Azevedo Koike |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Del Valle,Paulo Roberto Milani,Cintia Brentani,Maria Mitzi Katayama,Maria Lucia Hirata Lyra,Eduardo Carneiro de Carraro,Dirce Maria Brentani,Helena Puga,Renato Lima,Leandro A. Rozenchan,Patricia Bortman Nunes,Bárbara dos Santos Góes,João Carlos Guedes Sampaio Folgueira,Maria Aparecida Azevedo Koike |
dc.subject.por.fl_str_mv |
bone marrow breast neoplasm fibroblast gene expression profiling lymph node mesenchymal stromal cells tumor microenvironment |
topic |
bone marrow breast neoplasm fibroblast gene expression profiling lymph node mesenchymal stromal cells tumor microenvironment |
description |
Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were overrepresented. In a biological validation set, NOTCH2 was confirmed to be more expressed in N+ (vs CAF) and ADCY2, HECTD1, HNMT, LOX, MACF1, SLC1A3 and USP16 more expressed in BM (vs CAF). Only small differences were observed in the transcriptional profiles of fibroblasts from the primary tumor and lymph node of breast cancer patients, whereas greater differences were observed between bone marrow stromal cells and the other two sites. These differences may reflect the activities of distinct differentiation programs. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572014000400002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.37 n.3 2014 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122385907056640 |