The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600801 |
Resumo: | Abstract Gynostemma yixingense, an important medicinal member of the Cucurbitaceae family, is an endemic herbaceous species distributed in East China. It is morphologically similar to the plants in the same genus, which resulted in some confusion in identification and application. Meanwhile, there are still some controversies in taxonomy. Herein, the complete chloroplast genome sequence of G. yixingense was obtained by Illumina paired-end sequencing technology and compared to other chloroplast genome sequences of congeneric species. The complete chloroplast genome of G. yixingense is 157,910 bp in length with 36.94% GC content and contains a large single-copy (LSC) region of 86,791 bp, a small single-copy (SSC) region of 18,635 bp and a pair of inverted repeat (IR) regions of 26,242 bp. The whole genome contains 133 unique genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes and one pseudogene. In addition, 74 simple sequence repeats (SSRs) were identified, most of which were A/T rich. The phylogenetic analysis indicated that G. yixingense had the closest relationship to G. laxiflorum. The result of this study provided an important theoretical basis for chloroplast genome and phylogenetic analysis of G. yixingense. |
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Genetics and Molecular Biology |
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The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric speciesChloroplast genome, CucurbitaceaeGynostemmaphylogenysimple sequence repeatsAbstract Gynostemma yixingense, an important medicinal member of the Cucurbitaceae family, is an endemic herbaceous species distributed in East China. It is morphologically similar to the plants in the same genus, which resulted in some confusion in identification and application. Meanwhile, there are still some controversies in taxonomy. Herein, the complete chloroplast genome sequence of G. yixingense was obtained by Illumina paired-end sequencing technology and compared to other chloroplast genome sequences of congeneric species. The complete chloroplast genome of G. yixingense is 157,910 bp in length with 36.94% GC content and contains a large single-copy (LSC) region of 86,791 bp, a small single-copy (SSC) region of 18,635 bp and a pair of inverted repeat (IR) regions of 26,242 bp. The whole genome contains 133 unique genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes and one pseudogene. In addition, 74 simple sequence repeats (SSRs) were identified, most of which were A/T rich. The phylogenetic analysis indicated that G. yixingense had the closest relationship to G. laxiflorum. The result of this study provided an important theoretical basis for chloroplast genome and phylogenetic analysis of G. yixingense.Sociedade Brasileira de Genética2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600801Genetics and Molecular Biology v.43 n.4 2020reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2020-0092info:eu-repo/semantics/openAccessWang,LongLu,GengyuLiu,HaoHuang,LijinJiang,WeiminLi,PingLu,Xueng2020-09-25T00:00:00Zoai:scielo:S1415-47572020000600801Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2020-09-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species |
title |
The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species |
spellingShingle |
The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species Wang,Long Chloroplast genome, Cucurbitaceae Gynostemma phylogeny simple sequence repeats |
title_short |
The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species |
title_full |
The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species |
title_fullStr |
The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species |
title_full_unstemmed |
The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species |
title_sort |
The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species |
author |
Wang,Long |
author_facet |
Wang,Long Lu,Gengyu Liu,Hao Huang,Lijin Jiang,Weimin Li,Ping Lu,Xu |
author_role |
author |
author2 |
Lu,Gengyu Liu,Hao Huang,Lijin Jiang,Weimin Li,Ping Lu,Xu |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Wang,Long Lu,Gengyu Liu,Hao Huang,Lijin Jiang,Weimin Li,Ping Lu,Xu |
dc.subject.por.fl_str_mv |
Chloroplast genome, Cucurbitaceae Gynostemma phylogeny simple sequence repeats |
topic |
Chloroplast genome, Cucurbitaceae Gynostemma phylogeny simple sequence repeats |
description |
Abstract Gynostemma yixingense, an important medicinal member of the Cucurbitaceae family, is an endemic herbaceous species distributed in East China. It is morphologically similar to the plants in the same genus, which resulted in some confusion in identification and application. Meanwhile, there are still some controversies in taxonomy. Herein, the complete chloroplast genome sequence of G. yixingense was obtained by Illumina paired-end sequencing technology and compared to other chloroplast genome sequences of congeneric species. The complete chloroplast genome of G. yixingense is 157,910 bp in length with 36.94% GC content and contains a large single-copy (LSC) region of 86,791 bp, a small single-copy (SSC) region of 18,635 bp and a pair of inverted repeat (IR) regions of 26,242 bp. The whole genome contains 133 unique genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes and one pseudogene. In addition, 74 simple sequence repeats (SSRs) were identified, most of which were A/T rich. The phylogenetic analysis indicated that G. yixingense had the closest relationship to G. laxiflorum. The result of this study provided an important theoretical basis for chloroplast genome and phylogenetic analysis of G. yixingense. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600801 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600801 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2020-0092 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.43 n.4 2020 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122390168469504 |