The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species

Detalhes bibliográficos
Autor(a) principal: Wang,Long
Data de Publicação: 2020
Outros Autores: Lu,Gengyu, Liu,Hao, Huang,Lijin, Jiang,Weimin, Li,Ping, Lu,Xu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600801
Resumo: Abstract Gynostemma yixingense, an important medicinal member of the Cucurbitaceae family, is an endemic herbaceous species distributed in East China. It is morphologically similar to the plants in the same genus, which resulted in some confusion in identification and application. Meanwhile, there are still some controversies in taxonomy. Herein, the complete chloroplast genome sequence of G. yixingense was obtained by Illumina paired-end sequencing technology and compared to other chloroplast genome sequences of congeneric species. The complete chloroplast genome of G. yixingense is 157,910 bp in length with 36.94% GC content and contains a large single-copy (LSC) region of 86,791 bp, a small single-copy (SSC) region of 18,635 bp and a pair of inverted repeat (IR) regions of 26,242 bp. The whole genome contains 133 unique genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes and one pseudogene. In addition, 74 simple sequence repeats (SSRs) were identified, most of which were A/T rich. The phylogenetic analysis indicated that G. yixingense had the closest relationship to G. laxiflorum. The result of this study provided an important theoretical basis for chloroplast genome and phylogenetic analysis of G. yixingense.
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spelling The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric speciesChloroplast genome, CucurbitaceaeGynostemmaphylogenysimple sequence repeatsAbstract Gynostemma yixingense, an important medicinal member of the Cucurbitaceae family, is an endemic herbaceous species distributed in East China. It is morphologically similar to the plants in the same genus, which resulted in some confusion in identification and application. Meanwhile, there are still some controversies in taxonomy. Herein, the complete chloroplast genome sequence of G. yixingense was obtained by Illumina paired-end sequencing technology and compared to other chloroplast genome sequences of congeneric species. The complete chloroplast genome of G. yixingense is 157,910 bp in length with 36.94% GC content and contains a large single-copy (LSC) region of 86,791 bp, a small single-copy (SSC) region of 18,635 bp and a pair of inverted repeat (IR) regions of 26,242 bp. The whole genome contains 133 unique genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes and one pseudogene. In addition, 74 simple sequence repeats (SSRs) were identified, most of which were A/T rich. The phylogenetic analysis indicated that G. yixingense had the closest relationship to G. laxiflorum. The result of this study provided an important theoretical basis for chloroplast genome and phylogenetic analysis of G. yixingense.Sociedade Brasileira de Genética2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600801Genetics and Molecular Biology v.43 n.4 2020reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2020-0092info:eu-repo/semantics/openAccessWang,LongLu,GengyuLiu,HaoHuang,LijinJiang,WeiminLi,PingLu,Xueng2020-09-25T00:00:00Zoai:scielo:S1415-47572020000600801Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2020-09-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species
title The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species
spellingShingle The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species
Wang,Long
Chloroplast genome, Cucurbitaceae
Gynostemma
phylogeny
simple sequence repeats
title_short The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species
title_full The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species
title_fullStr The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species
title_full_unstemmed The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species
title_sort The complete chloroplast genome sequence of Gynostemma yixingense and comparative analysis with congeneric species
author Wang,Long
author_facet Wang,Long
Lu,Gengyu
Liu,Hao
Huang,Lijin
Jiang,Weimin
Li,Ping
Lu,Xu
author_role author
author2 Lu,Gengyu
Liu,Hao
Huang,Lijin
Jiang,Weimin
Li,Ping
Lu,Xu
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wang,Long
Lu,Gengyu
Liu,Hao
Huang,Lijin
Jiang,Weimin
Li,Ping
Lu,Xu
dc.subject.por.fl_str_mv Chloroplast genome, Cucurbitaceae
Gynostemma
phylogeny
simple sequence repeats
topic Chloroplast genome, Cucurbitaceae
Gynostemma
phylogeny
simple sequence repeats
description Abstract Gynostemma yixingense, an important medicinal member of the Cucurbitaceae family, is an endemic herbaceous species distributed in East China. It is morphologically similar to the plants in the same genus, which resulted in some confusion in identification and application. Meanwhile, there are still some controversies in taxonomy. Herein, the complete chloroplast genome sequence of G. yixingense was obtained by Illumina paired-end sequencing technology and compared to other chloroplast genome sequences of congeneric species. The complete chloroplast genome of G. yixingense is 157,910 bp in length with 36.94% GC content and contains a large single-copy (LSC) region of 86,791 bp, a small single-copy (SSC) region of 18,635 bp and a pair of inverted repeat (IR) regions of 26,242 bp. The whole genome contains 133 unique genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes and one pseudogene. In addition, 74 simple sequence repeats (SSRs) were identified, most of which were A/T rich. The phylogenetic analysis indicated that G. yixingense had the closest relationship to G. laxiflorum. The result of this study provided an important theoretical basis for chloroplast genome and phylogenetic analysis of G. yixingense.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600801
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600801
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2020-0092
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.43 n.4 2020
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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instname_str Sociedade Brasileira de Genética (SBG)
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