A simple procedure for rehybridization of nuclei analyzed previously by fish

Detalhes bibliográficos
Autor(a) principal: Carvalho-Salles,Andréa Borduchi
Data de Publicação: 1999
Outros Autores: Barril,Nilce, Tajara,Eloiza Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200006
Resumo: We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available.
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spelling A simple procedure for rehybridization of nuclei analyzed previously by fishWe present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available.Sociedade Brasileira de Genética1999-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200006Genetics and Molecular Biology v.22 n.2 1999reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571999000200006info:eu-repo/semantics/openAccessCarvalho-Salles,Andréa BorduchiBarril,NilceTajara,Eloiza Helenaeng1999-08-27T00:00:00Zoai:scielo:S1415-47571999000200006Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-08-27T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv A simple procedure for rehybridization of nuclei analyzed previously by fish
title A simple procedure for rehybridization of nuclei analyzed previously by fish
spellingShingle A simple procedure for rehybridization of nuclei analyzed previously by fish
Carvalho-Salles,Andréa Borduchi
title_short A simple procedure for rehybridization of nuclei analyzed previously by fish
title_full A simple procedure for rehybridization of nuclei analyzed previously by fish
title_fullStr A simple procedure for rehybridization of nuclei analyzed previously by fish
title_full_unstemmed A simple procedure for rehybridization of nuclei analyzed previously by fish
title_sort A simple procedure for rehybridization of nuclei analyzed previously by fish
author Carvalho-Salles,Andréa Borduchi
author_facet Carvalho-Salles,Andréa Borduchi
Barril,Nilce
Tajara,Eloiza Helena
author_role author
author2 Barril,Nilce
Tajara,Eloiza Helena
author2_role author
author
dc.contributor.author.fl_str_mv Carvalho-Salles,Andréa Borduchi
Barril,Nilce
Tajara,Eloiza Helena
description We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available.
publishDate 1999
dc.date.none.fl_str_mv 1999-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47571999000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.22 n.2 1999
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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