Genetic and epigenetic effects of salinity on in vitro growth of barley

Detalhes bibliográficos
Autor(a) principal: Demirkiran,Aykut
Data de Publicação: 2013
Outros Autores: Marakli,Sevgi, Temel,Aslihan, Gozukirmizi,Nermin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400016
Resumo: Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley.
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spelling Genetic and epigenetic effects of salinity on in vitro growth of barleyHordeum vulgareSalt stressTissue cultureRAPDCRED-RAMorphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley.Sociedade Brasileira de Genética2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400016Genetics and Molecular Biology v.36 n.4 2013reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572013000400016info:eu-repo/semantics/openAccessDemirkiran,AykutMarakli,SevgiTemel,AslihanGozukirmizi,Nermineng2015-07-28T00:00:00Zoai:scielo:S1415-47572013000400016Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2015-07-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Genetic and epigenetic effects of salinity on in vitro growth of barley
title Genetic and epigenetic effects of salinity on in vitro growth of barley
spellingShingle Genetic and epigenetic effects of salinity on in vitro growth of barley
Demirkiran,Aykut
Hordeum vulgare
Salt stress
Tissue culture
RAPD
CRED-RA
title_short Genetic and epigenetic effects of salinity on in vitro growth of barley
title_full Genetic and epigenetic effects of salinity on in vitro growth of barley
title_fullStr Genetic and epigenetic effects of salinity on in vitro growth of barley
title_full_unstemmed Genetic and epigenetic effects of salinity on in vitro growth of barley
title_sort Genetic and epigenetic effects of salinity on in vitro growth of barley
author Demirkiran,Aykut
author_facet Demirkiran,Aykut
Marakli,Sevgi
Temel,Aslihan
Gozukirmizi,Nermin
author_role author
author2 Marakli,Sevgi
Temel,Aslihan
Gozukirmizi,Nermin
author2_role author
author
author
dc.contributor.author.fl_str_mv Demirkiran,Aykut
Marakli,Sevgi
Temel,Aslihan
Gozukirmizi,Nermin
dc.subject.por.fl_str_mv Hordeum vulgare
Salt stress
Tissue culture
RAPD
CRED-RA
topic Hordeum vulgare
Salt stress
Tissue culture
RAPD
CRED-RA
description Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572013000400016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.36 n.4 2013
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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