DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars

Detalhes bibliográficos
Autor(a) principal: Sandhu,S.S.
Data de Publicação: 2003
Outros Autores: Colombo,Carlos, Bastos,Cândido R., Siqueira,Walter J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011
Resumo: Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs.
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spelling DNA tagging of blast resistant gene(s) in three Brazilian rice cultivarsriceSCARarbitrary primersPyricularia oryzaeRice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs.Sociedade Brasileira de Genética2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011Genetics and Molecular Biology v.26 n.4 2003reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572003000400011info:eu-repo/semantics/openAccessSandhu,S.S.Colombo,CarlosBastos,Cândido R.Siqueira,Walter J.eng2004-04-06T00:00:00Zoai:scielo:S1415-47572003000400011Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2004-04-06T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
spellingShingle DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
Sandhu,S.S.
rice
SCAR
arbitrary primers
Pyricularia oryzae
title_short DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title_full DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title_fullStr DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title_full_unstemmed DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
title_sort DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
author Sandhu,S.S.
author_facet Sandhu,S.S.
Colombo,Carlos
Bastos,Cândido R.
Siqueira,Walter J.
author_role author
author2 Colombo,Carlos
Bastos,Cândido R.
Siqueira,Walter J.
author2_role author
author
author
dc.contributor.author.fl_str_mv Sandhu,S.S.
Colombo,Carlos
Bastos,Cândido R.
Siqueira,Walter J.
dc.subject.por.fl_str_mv rice
SCAR
arbitrary primers
Pyricularia oryzae
topic rice
SCAR
arbitrary primers
Pyricularia oryzae
description Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572003000400011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.26 n.4 2003
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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