Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants

Detalhes bibliográficos
Autor(a) principal: Falco,J.R.P.
Data de Publicação: 1999
Outros Autores: Mello,M.L.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200010
Resumo: The critical electrolyte concentrations (CEC) of sperm chromatin from animal species known or suspected to contain histone H1 variants were compared by examining the affinity of their DNA-protein complexes for toluidine blue in the presence of Mg2+. Bullfrog, sea urchin, bee and bumblebee spermatozoa were studied. The CEC for Rana catesbeiana and two sea urchin species were similar to that of histone H5-containing chromatin from chicken erythrocytes, thus confirming the biochemical and structural similarities of these DNA-protein complexes. The CEC for bees and the bumblebee, Bombus atratus, showed no particular phylogenetic relationship. We concluded that the CEC of histone H1-containing sperm cell chromatin is a useful indicator of variability in DNA-protein complexes but is of little phylogenetic value.
id SBG-1_c9be78d6f2b61630a89488e6f8a5fe72
oai_identifier_str oai:scielo:S1415-47571999000200010
network_acronym_str SBG-1
network_name_str Genetics and Molecular Biology
repository_id_str
spelling Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variantsThe critical electrolyte concentrations (CEC) of sperm chromatin from animal species known or suspected to contain histone H1 variants were compared by examining the affinity of their DNA-protein complexes for toluidine blue in the presence of Mg2+. Bullfrog, sea urchin, bee and bumblebee spermatozoa were studied. The CEC for Rana catesbeiana and two sea urchin species were similar to that of histone H5-containing chromatin from chicken erythrocytes, thus confirming the biochemical and structural similarities of these DNA-protein complexes. The CEC for bees and the bumblebee, Bombus atratus, showed no particular phylogenetic relationship. We concluded that the CEC of histone H1-containing sperm cell chromatin is a useful indicator of variability in DNA-protein complexes but is of little phylogenetic value.Sociedade Brasileira de Genética1999-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200010Genetics and Molecular Biology v.22 n.2 1999reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571999000200010info:eu-repo/semantics/openAccessFalco,J.R.P.Mello,M.L.S.eng1999-08-26T00:00:00Zoai:scielo:S1415-47571999000200010Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-08-26T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
title Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
spellingShingle Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
Falco,J.R.P.
title_short Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
title_full Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
title_fullStr Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
title_full_unstemmed Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
title_sort Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
author Falco,J.R.P.
author_facet Falco,J.R.P.
Mello,M.L.S.
author_role author
author2 Mello,M.L.S.
author2_role author
dc.contributor.author.fl_str_mv Falco,J.R.P.
Mello,M.L.S.
description The critical electrolyte concentrations (CEC) of sperm chromatin from animal species known or suspected to contain histone H1 variants were compared by examining the affinity of their DNA-protein complexes for toluidine blue in the presence of Mg2+. Bullfrog, sea urchin, bee and bumblebee spermatozoa were studied. The CEC for Rana catesbeiana and two sea urchin species were similar to that of histone H5-containing chromatin from chicken erythrocytes, thus confirming the biochemical and structural similarities of these DNA-protein complexes. The CEC for bees and the bumblebee, Bombus atratus, showed no particular phylogenetic relationship. We concluded that the CEC of histone H1-containing sperm cell chromatin is a useful indicator of variability in DNA-protein complexes but is of little phylogenetic value.
publishDate 1999
dc.date.none.fl_str_mv 1999-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47571999000200010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.22 n.2 1999
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
_version_ 1752122377349627904

Registros relacionados