Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)

Detalhes bibliográficos
Autor(a) principal: Silva,Jorge A.G. da
Data de Publicação: 2001
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100021
Resumo: Expressed sequence tags (ESTs) in the sugarcane (Saccharum spp) database (SUCEST) were electronically searched and 402 microsatellites identified. Various dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were found, with these being more frequently observed in ESTs obtained from flower cDNA libraries. PCR primers were designed for 20 of these SSRs and were tested on eight sugarcane genotypes, the sequences of these primers and a list of known sugarcane genes containing SSR motifs being presented in this paper. Polymorphisms were evident both at the cultivar level and between Saccharum species. These results show that EST-derived SSRs in Saccharum species are useful because they are polymorphic and transferable. The large number of microsatellites that will eventually be available from the SUCEST database (containing 295,000 submitted reads) will have many potential applications in linkage mapping and the planning of crosses.
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spelling Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)Expressed sequence tags (ESTs) in the sugarcane (Saccharum spp) database (SUCEST) were electronically searched and 402 microsatellites identified. Various dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were found, with these being more frequently observed in ESTs obtained from flower cDNA libraries. PCR primers were designed for 20 of these SSRs and were tested on eight sugarcane genotypes, the sequences of these primers and a list of known sugarcane genes containing SSR motifs being presented in this paper. Polymorphisms were evident both at the cultivar level and between Saccharum species. These results show that EST-derived SSRs in Saccharum species are useful because they are polymorphic and transferable. The large number of microsatellites that will eventually be available from the SUCEST database (containing 295,000 submitted reads) will have many potential applications in linkage mapping and the planning of crosses.Sociedade Brasileira de Genética2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100021Genetics and Molecular Biology v.24 n.1-4 2001reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572001000100021info:eu-repo/semantics/openAccessSilva,Jorge A.G. daeng2002-06-27T00:00:00Zoai:scielo:S1415-47572001000100021Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2002-06-27T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
title Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
spellingShingle Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
Silva,Jorge A.G. da
title_short Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
title_full Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
title_fullStr Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
title_full_unstemmed Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
title_sort Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
author Silva,Jorge A.G. da
author_facet Silva,Jorge A.G. da
author_role author
dc.contributor.author.fl_str_mv Silva,Jorge A.G. da
description Expressed sequence tags (ESTs) in the sugarcane (Saccharum spp) database (SUCEST) were electronically searched and 402 microsatellites identified. Various dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were found, with these being more frequently observed in ESTs obtained from flower cDNA libraries. PCR primers were designed for 20 of these SSRs and were tested on eight sugarcane genotypes, the sequences of these primers and a list of known sugarcane genes containing SSR motifs being presented in this paper. Polymorphisms were evident both at the cultivar level and between Saccharum species. These results show that EST-derived SSRs in Saccharum species are useful because they are polymorphic and transferable. The large number of microsatellites that will eventually be available from the SUCEST database (containing 295,000 submitted reads) will have many potential applications in linkage mapping and the planning of crosses.
publishDate 2001
dc.date.none.fl_str_mv 2001-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572001000100021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.24 n.1-4 2001
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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instname_str Sociedade Brasileira de Genética (SBG)
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reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
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