Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro

Detalhes bibliográficos
Autor(a) principal: Araújo,Maria Cristina P.
Data de Publicação: 1998
Outros Autores: Dias,Francisca da Luz, Cecchi,Andréa O., Antunes,Lusânia M.G., Takahashi,Catarina S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300021
Resumo: Combined radiation and antineoplastic drug treatment have important applications in cancer therapy. In the present work, an evaluation was made of two known topoisomerase II inhibitors, doxorubicin (DXR) and mitoxantrone (MXN), with g-radiation. The effects of DXR or MXN on g-radiation-induced chromosome aberrations in Chinese hamster ovary (CHO) cells were analyzed. Two concentrations of each drug, 0.5 and 1.0 µg/ml DXR, and 0.02 and 0.04 µg/ml MXN, were applied in combination with two doses of g-radiation (20 and 40 cGy). A significant potentiating effect on chromosomal aberrations was observed in CHO cells exposed to 1.0 µg/ml DXR plus 40 cGy. In the other tests, the combination of g-radiation with DXR or MXN gave approximately additive effects. Reduced mitotic indices reflected higher toxicity of the drugs when combined with radiation.
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spelling Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitroCombined radiation and antineoplastic drug treatment have important applications in cancer therapy. In the present work, an evaluation was made of two known topoisomerase II inhibitors, doxorubicin (DXR) and mitoxantrone (MXN), with g-radiation. The effects of DXR or MXN on g-radiation-induced chromosome aberrations in Chinese hamster ovary (CHO) cells were analyzed. Two concentrations of each drug, 0.5 and 1.0 µg/ml DXR, and 0.02 and 0.04 µg/ml MXN, were applied in combination with two doses of g-radiation (20 and 40 cGy). A significant potentiating effect on chromosomal aberrations was observed in CHO cells exposed to 1.0 µg/ml DXR plus 40 cGy. In the other tests, the combination of g-radiation with DXR or MXN gave approximately additive effects. Reduced mitotic indices reflected higher toxicity of the drugs when combined with radiation.Sociedade Brasileira de Genética1998-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300021Genetics and Molecular Biology v.21 n.3 1998reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571998000300021info:eu-repo/semantics/openAccessAraújo,Maria Cristina P.Dias,Francisca da LuzCecchi,Andréa O.Antunes,Lusânia M.G.Takahashi,Catarina S.eng1999-02-23T00:00:00Zoai:scielo:S1415-47571998000300021Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-02-23T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro
title Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro
spellingShingle Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro
Araújo,Maria Cristina P.
title_short Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro
title_full Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro
title_fullStr Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro
title_full_unstemmed Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro
title_sort Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro
author Araújo,Maria Cristina P.
author_facet Araújo,Maria Cristina P.
Dias,Francisca da Luz
Cecchi,Andréa O.
Antunes,Lusânia M.G.
Takahashi,Catarina S.
author_role author
author2 Dias,Francisca da Luz
Cecchi,Andréa O.
Antunes,Lusânia M.G.
Takahashi,Catarina S.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Araújo,Maria Cristina P.
Dias,Francisca da Luz
Cecchi,Andréa O.
Antunes,Lusânia M.G.
Takahashi,Catarina S.
description Combined radiation and antineoplastic drug treatment have important applications in cancer therapy. In the present work, an evaluation was made of two known topoisomerase II inhibitors, doxorubicin (DXR) and mitoxantrone (MXN), with g-radiation. The effects of DXR or MXN on g-radiation-induced chromosome aberrations in Chinese hamster ovary (CHO) cells were analyzed. Two concentrations of each drug, 0.5 and 1.0 µg/ml DXR, and 0.02 and 0.04 µg/ml MXN, were applied in combination with two doses of g-radiation (20 and 40 cGy). A significant potentiating effect on chromosomal aberrations was observed in CHO cells exposed to 1.0 µg/ml DXR plus 40 cGy. In the other tests, the combination of g-radiation with DXR or MXN gave approximately additive effects. Reduced mitotic indices reflected higher toxicity of the drugs when combined with radiation.
publishDate 1998
dc.date.none.fl_str_mv 1998-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47571998000300021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.21 n.3 1998
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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