Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines

Detalhes bibliográficos
Autor(a) principal: Yamada,Hiroya
Data de Publicação: 2011
Outros Autores: Takano,Toru, Matsuzuka,Fumio, Watanabe,Mikio, Miyauchi,Akira, Iwatani,Yoshinori
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100002
Resumo: Thyroid transcription factor-1 (TTF-1, NKX2-1) is a homeodomain-containing transcriptional factor that binds to and activates the promoters of thyroid and lung-specific genes, such as thyroglobulin, thyroid peroxidase, and thyroid stimulating hormone receptor. TTF-1 is known to play a key role in the development of the thyroid. However, the precise mechanism of TTF-1 gene transcription in human thyroid cells has not been studied. The expression of transcriptional activity in various lengths of the 5'-flanking region of the human TTF -1 gene was studied in TTF-1 positive and negative human thyroid cell lines. Increased transcriptional activity was observed in thyroid cell lines containing plasmids that coded for a sequence proximal to the transcription start site of exon 1 of the TTF-1 gene. However, we did not observe any difference in promoter activity in the region up to -2.6 kb from the proximal transcription start site of the TTF-1 gene between TTF-1 positive and negative cells. These results suggest that the proximal 5'-flanking region of the human TTF -1 gene does not contain sufficient cis-active regulatory information to direct gene expression in thyroid cells,and that other cis-or trans-acting factors participate in the thyroid specific gene expression of TTF-1.
id SBG-1_de1f4946a6b8d303afc77263e8877b9b
oai_identifier_str oai:scielo:S1415-47572011000100002
network_acronym_str SBG-1
network_name_str Genetics and Molecular Biology
repository_id_str
spelling Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell linesthyroidTTF-1transcriptionhuman cell linestem cellThyroid transcription factor-1 (TTF-1, NKX2-1) is a homeodomain-containing transcriptional factor that binds to and activates the promoters of thyroid and lung-specific genes, such as thyroglobulin, thyroid peroxidase, and thyroid stimulating hormone receptor. TTF-1 is known to play a key role in the development of the thyroid. However, the precise mechanism of TTF-1 gene transcription in human thyroid cells has not been studied. The expression of transcriptional activity in various lengths of the 5'-flanking region of the human TTF -1 gene was studied in TTF-1 positive and negative human thyroid cell lines. Increased transcriptional activity was observed in thyroid cell lines containing plasmids that coded for a sequence proximal to the transcription start site of exon 1 of the TTF-1 gene. However, we did not observe any difference in promoter activity in the region up to -2.6 kb from the proximal transcription start site of the TTF-1 gene between TTF-1 positive and negative cells. These results suggest that the proximal 5'-flanking region of the human TTF -1 gene does not contain sufficient cis-active regulatory information to direct gene expression in thyroid cells,and that other cis-or trans-acting factors participate in the thyroid specific gene expression of TTF-1.Sociedade Brasileira de Genética2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100002Genetics and Molecular Biology v.34 n.1 2011reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572010005000109info:eu-repo/semantics/openAccessYamada,HiroyaTakano,ToruMatsuzuka,FumioWatanabe,MikioMiyauchi,AkiraIwatani,Yoshinorieng2011-01-28T00:00:00Zoai:scielo:S1415-47572011000100002Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2011-01-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
title Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
spellingShingle Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
Yamada,Hiroya
thyroid
TTF-1
transcription
human cell line
stem cell
title_short Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
title_full Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
title_fullStr Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
title_full_unstemmed Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
title_sort Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
author Yamada,Hiroya
author_facet Yamada,Hiroya
Takano,Toru
Matsuzuka,Fumio
Watanabe,Mikio
Miyauchi,Akira
Iwatani,Yoshinori
author_role author
author2 Takano,Toru
Matsuzuka,Fumio
Watanabe,Mikio
Miyauchi,Akira
Iwatani,Yoshinori
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Yamada,Hiroya
Takano,Toru
Matsuzuka,Fumio
Watanabe,Mikio
Miyauchi,Akira
Iwatani,Yoshinori
dc.subject.por.fl_str_mv thyroid
TTF-1
transcription
human cell line
stem cell
topic thyroid
TTF-1
transcription
human cell line
stem cell
description Thyroid transcription factor-1 (TTF-1, NKX2-1) is a homeodomain-containing transcriptional factor that binds to and activates the promoters of thyroid and lung-specific genes, such as thyroglobulin, thyroid peroxidase, and thyroid stimulating hormone receptor. TTF-1 is known to play a key role in the development of the thyroid. However, the precise mechanism of TTF-1 gene transcription in human thyroid cells has not been studied. The expression of transcriptional activity in various lengths of the 5'-flanking region of the human TTF -1 gene was studied in TTF-1 positive and negative human thyroid cell lines. Increased transcriptional activity was observed in thyroid cell lines containing plasmids that coded for a sequence proximal to the transcription start site of exon 1 of the TTF-1 gene. However, we did not observe any difference in promoter activity in the region up to -2.6 kb from the proximal transcription start site of the TTF-1 gene between TTF-1 positive and negative cells. These results suggest that the proximal 5'-flanking region of the human TTF -1 gene does not contain sufficient cis-active regulatory information to direct gene expression in thyroid cells,and that other cis-or trans-acting factors participate in the thyroid specific gene expression of TTF-1.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572010005000109
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.34 n.1 2011
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
_version_ 1752122383649472512

Registros relacionados