Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug

Detalhes bibliográficos
Autor(a) principal: Mello,Maria Luiza S.
Data de Publicação: 2000
Outros Autores: Maria,Sílvya S., Tavares,Maria Cristina H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200011
Resumo: The survival of Triatoma infestans and of somatic cells from this species is affected by heat shock. In this study, we examined the cell death responses of male germ line cells from 5th instar nymphs 7 and 30 days after heat shock exposure (40ºC, 1 h). The preparations were stained by the Feulgen reaction and the TUNEL immunocytochemical assay. Apoptosis was elicited by heat shock, with positive TUNEL responses in spermatogonial chromatin and chromosomes, spermatids and sperm cells. Spermatogonia were most affected seven days after the shock whereas some spermatids and sperm cells exhibited DNA fragmentation only thirty days after heat shock. The rate of cell death varied among the cells. In some cases, cellular differentiation was unaffected by heat shock, with DNA fragmentation occurring towards the end of spermatogenesis.
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spelling Heat shock-induced apoptosis in germ line cells of Triatoma infestans KlugThe survival of Triatoma infestans and of somatic cells from this species is affected by heat shock. In this study, we examined the cell death responses of male germ line cells from 5th instar nymphs 7 and 30 days after heat shock exposure (40ºC, 1 h). The preparations were stained by the Feulgen reaction and the TUNEL immunocytochemical assay. Apoptosis was elicited by heat shock, with positive TUNEL responses in spermatogonial chromatin and chromosomes, spermatids and sperm cells. Spermatogonia were most affected seven days after the shock whereas some spermatids and sperm cells exhibited DNA fragmentation only thirty days after heat shock. The rate of cell death varied among the cells. In some cases, cellular differentiation was unaffected by heat shock, with DNA fragmentation occurring towards the end of spermatogenesis.Sociedade Brasileira de Genética2000-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200011Genetics and Molecular Biology v.23 n.2 2000reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572000000200011info:eu-repo/semantics/openAccessMello,Maria Luiza S.Maria,Sílvya S.Tavares,Maria Cristina H.eng2000-09-22T00:00:00Zoai:scielo:S1415-47572000000200011Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2000-09-22T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug
title Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug
spellingShingle Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug
Mello,Maria Luiza S.
title_short Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug
title_full Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug
title_fullStr Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug
title_full_unstemmed Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug
title_sort Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug
author Mello,Maria Luiza S.
author_facet Mello,Maria Luiza S.
Maria,Sílvya S.
Tavares,Maria Cristina H.
author_role author
author2 Maria,Sílvya S.
Tavares,Maria Cristina H.
author2_role author
author
dc.contributor.author.fl_str_mv Mello,Maria Luiza S.
Maria,Sílvya S.
Tavares,Maria Cristina H.
description The survival of Triatoma infestans and of somatic cells from this species is affected by heat shock. In this study, we examined the cell death responses of male germ line cells from 5th instar nymphs 7 and 30 days after heat shock exposure (40ºC, 1 h). The preparations were stained by the Feulgen reaction and the TUNEL immunocytochemical assay. Apoptosis was elicited by heat shock, with positive TUNEL responses in spermatogonial chromatin and chromosomes, spermatids and sperm cells. Spermatogonia were most affected seven days after the shock whereas some spermatids and sperm cells exhibited DNA fragmentation only thirty days after heat shock. The rate of cell death varied among the cells. In some cases, cellular differentiation was unaffected by heat shock, with DNA fragmentation occurring towards the end of spermatogenesis.
publishDate 2000
dc.date.none.fl_str_mv 2000-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572000000200011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.23 n.2 2000
reponame:Genetics and Molecular Biology
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reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
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