Characterization analysis and polymorphism detection of the porcine Myd88 gene

Detalhes bibliográficos
Autor(a) principal: Li,Xinyun
Data de Publicação: 2009
Outros Autores: Liu,Huazhen, Shulin,Yang, Tang,Zhonglin, Ma,Yuehui, Chu,Mingxing, Li,Kui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200015
Resumo: The myeloid differentiation primary response protein 88 (Myd88) is an essential adaptor protein, which mediates in all Toll-like receptor (TLR) members signal transduction, except for TLR3. In this study, the 4464 bp genomic sequence of porcine Myd88 was first isolated, whereupon tissue distribution, chromosome mapping and single nucleotide polymorphism (SNP) were analyzed. Our results revealed that porcine Myd88 gene, which was located at chromosome 13 linked with marker S0288 (distance = 40 cR; LOD = 8.66), was widely expressed in all the examined tissues. There were 16 potential SNPs in the isolated genome fragment. SNP 797T/C in the first intron was studied, with no significant association being found between the genotype and immune traits in pigs (p > 0.05). The porcine Myd88 protein contained both the death domain (DD) and the Toll/IL-1 receptor domain (TIR). Leu residues, essential for its structure, were the most abundant encountered in the DD. The TIR contained two conserved motifs which may play important roles in the Myd88 function.
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spelling Characterization analysis and polymorphism detection of the porcine Myd88 geneMyd88TLRpolymorphismpigchromosome mappingThe myeloid differentiation primary response protein 88 (Myd88) is an essential adaptor protein, which mediates in all Toll-like receptor (TLR) members signal transduction, except for TLR3. In this study, the 4464 bp genomic sequence of porcine Myd88 was first isolated, whereupon tissue distribution, chromosome mapping and single nucleotide polymorphism (SNP) were analyzed. Our results revealed that porcine Myd88 gene, which was located at chromosome 13 linked with marker S0288 (distance = 40 cR; LOD = 8.66), was widely expressed in all the examined tissues. There were 16 potential SNPs in the isolated genome fragment. SNP 797T/C in the first intron was studied, with no significant association being found between the genotype and immune traits in pigs (p > 0.05). The porcine Myd88 protein contained both the death domain (DD) and the Toll/IL-1 receptor domain (TIR). Leu residues, essential for its structure, were the most abundant encountered in the DD. The TIR contained two conserved motifs which may play important roles in the Myd88 function.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200015Genetics and Molecular Biology v.32 n.2 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009000200015info:eu-repo/semantics/openAccessLi,XinyunLiu,HuazhenShulin,YangTang,ZhonglinMa,YuehuiChu,MingxingLi,Kuieng2009-05-25T00:00:00Zoai:scielo:S1415-47572009000200015Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-05-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Characterization analysis and polymorphism detection of the porcine Myd88 gene
title Characterization analysis and polymorphism detection of the porcine Myd88 gene
spellingShingle Characterization analysis and polymorphism detection of the porcine Myd88 gene
Li,Xinyun
Myd88
TLR
polymorphism
pig
chromosome mapping
title_short Characterization analysis and polymorphism detection of the porcine Myd88 gene
title_full Characterization analysis and polymorphism detection of the porcine Myd88 gene
title_fullStr Characterization analysis and polymorphism detection of the porcine Myd88 gene
title_full_unstemmed Characterization analysis and polymorphism detection of the porcine Myd88 gene
title_sort Characterization analysis and polymorphism detection of the porcine Myd88 gene
author Li,Xinyun
author_facet Li,Xinyun
Liu,Huazhen
Shulin,Yang
Tang,Zhonglin
Ma,Yuehui
Chu,Mingxing
Li,Kui
author_role author
author2 Liu,Huazhen
Shulin,Yang
Tang,Zhonglin
Ma,Yuehui
Chu,Mingxing
Li,Kui
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Li,Xinyun
Liu,Huazhen
Shulin,Yang
Tang,Zhonglin
Ma,Yuehui
Chu,Mingxing
Li,Kui
dc.subject.por.fl_str_mv Myd88
TLR
polymorphism
pig
chromosome mapping
topic Myd88
TLR
polymorphism
pig
chromosome mapping
description The myeloid differentiation primary response protein 88 (Myd88) is an essential adaptor protein, which mediates in all Toll-like receptor (TLR) members signal transduction, except for TLR3. In this study, the 4464 bp genomic sequence of porcine Myd88 was first isolated, whereupon tissue distribution, chromosome mapping and single nucleotide polymorphism (SNP) were analyzed. Our results revealed that porcine Myd88 gene, which was located at chromosome 13 linked with marker S0288 (distance = 40 cR; LOD = 8.66), was widely expressed in all the examined tissues. There were 16 potential SNPs in the isolated genome fragment. SNP 797T/C in the first intron was studied, with no significant association being found between the genotype and immune traits in pigs (p > 0.05). The porcine Myd88 protein contained both the death domain (DD) and the Toll/IL-1 receptor domain (TIR). Leu residues, essential for its structure, were the most abundant encountered in the DD. The TIR contained two conserved motifs which may play important roles in the Myd88 function.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572009000200015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.32 n.2 2009
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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