Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500402 |
Resumo: | Abstract Banana tree bacterial wilt is caused by the Ralstonia solanacearum Moko ecotype. These strains vary in their symptom progression in banana, and are classified as typical Moko variants (phylotype IIA and IIB strains from across Central and South America), Bugtok variant (Philippines), and Sergipe facies (the states of Sergipe and Alagoas, Brazil). This study used comparative genomic and phylogenomic approaches to identify a correlation between the symptom progression of the Moko ecotypes based on the analysis of 23 available genomes. Average nucleotide identity and in silico DNA-DNA hybridization revealed a high correlation (>96% and >78%, respectively) between the genomes of Moko variants. Pan-genome analysis identified 21.3% of inheritable regions between representatives of the typical Moko and Sergipe facies variants, which could be traced to an abundance of exclusive homolog clusters. Moko ecotype genomes shared 1,951 orthologous genes, but representatives with typical symptoms did not display unique orthologues. Moreover, Bugtok disease and Sergipe facies genomes did not share any unique genes, suggesting convergent evolution to a shared symptom progression. Overall, genomic and phylogenomic analyses were insufficient to differentiate the Moko variants based on symptom progression. |
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Genetics and Molecular Biology |
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Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variantsGenomicsMusa sppSergipe faciesBugtoksymptomatologyAbstract Banana tree bacterial wilt is caused by the Ralstonia solanacearum Moko ecotype. These strains vary in their symptom progression in banana, and are classified as typical Moko variants (phylotype IIA and IIB strains from across Central and South America), Bugtok variant (Philippines), and Sergipe facies (the states of Sergipe and Alagoas, Brazil). This study used comparative genomic and phylogenomic approaches to identify a correlation between the symptom progression of the Moko ecotypes based on the analysis of 23 available genomes. Average nucleotide identity and in silico DNA-DNA hybridization revealed a high correlation (>96% and >78%, respectively) between the genomes of Moko variants. Pan-genome analysis identified 21.3% of inheritable regions between representatives of the typical Moko and Sergipe facies variants, which could be traced to an abundance of exclusive homolog clusters. Moko ecotype genomes shared 1,951 orthologous genes, but representatives with typical symptoms did not display unique orthologues. Moreover, Bugtok disease and Sergipe facies genomes did not share any unique genes, suggesting convergent evolution to a shared symptom progression. Overall, genomic and phylogenomic analyses were insufficient to differentiate the Moko variants based on symptom progression.Sociedade Brasileira de Genética2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500402Genetics and Molecular Biology v.45 n.4 2022reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2022-0038info:eu-repo/semantics/openAccessPais,Ana Karolina LeiteSantos,Leandro Victor Silva dosAlbuquerque,Greecy Mirian RodriguesFarias,Antonio Roberto Gomes deSilva Junior,Wilson JoséBalbino,Valdir de QueirozSilva,Adriano Márcio FreireGama,Marco Aurelio Siqueira daSouza,Elineide Barbosa deeng2022-11-29T00:00:00Zoai:scielo:S1415-47572022000500402Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2022-11-29T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants |
title |
Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants |
spellingShingle |
Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants Pais,Ana Karolina Leite Genomics Musa spp Sergipe facies Bugtok symptomatology |
title_short |
Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants |
title_full |
Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants |
title_fullStr |
Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants |
title_full_unstemmed |
Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants |
title_sort |
Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants |
author |
Pais,Ana Karolina Leite |
author_facet |
Pais,Ana Karolina Leite Santos,Leandro Victor Silva dos Albuquerque,Greecy Mirian Rodrigues Farias,Antonio Roberto Gomes de Silva Junior,Wilson José Balbino,Valdir de Queiroz Silva,Adriano Márcio Freire Gama,Marco Aurelio Siqueira da Souza,Elineide Barbosa de |
author_role |
author |
author2 |
Santos,Leandro Victor Silva dos Albuquerque,Greecy Mirian Rodrigues Farias,Antonio Roberto Gomes de Silva Junior,Wilson José Balbino,Valdir de Queiroz Silva,Adriano Márcio Freire Gama,Marco Aurelio Siqueira da Souza,Elineide Barbosa de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pais,Ana Karolina Leite Santos,Leandro Victor Silva dos Albuquerque,Greecy Mirian Rodrigues Farias,Antonio Roberto Gomes de Silva Junior,Wilson José Balbino,Valdir de Queiroz Silva,Adriano Márcio Freire Gama,Marco Aurelio Siqueira da Souza,Elineide Barbosa de |
dc.subject.por.fl_str_mv |
Genomics Musa spp Sergipe facies Bugtok symptomatology |
topic |
Genomics Musa spp Sergipe facies Bugtok symptomatology |
description |
Abstract Banana tree bacterial wilt is caused by the Ralstonia solanacearum Moko ecotype. These strains vary in their symptom progression in banana, and are classified as typical Moko variants (phylotype IIA and IIB strains from across Central and South America), Bugtok variant (Philippines), and Sergipe facies (the states of Sergipe and Alagoas, Brazil). This study used comparative genomic and phylogenomic approaches to identify a correlation between the symptom progression of the Moko ecotypes based on the analysis of 23 available genomes. Average nucleotide identity and in silico DNA-DNA hybridization revealed a high correlation (>96% and >78%, respectively) between the genomes of Moko variants. Pan-genome analysis identified 21.3% of inheritable regions between representatives of the typical Moko and Sergipe facies variants, which could be traced to an abundance of exclusive homolog clusters. Moko ecotype genomes shared 1,951 orthologous genes, but representatives with typical symptoms did not display unique orthologues. Moreover, Bugtok disease and Sergipe facies genomes did not share any unique genes, suggesting convergent evolution to a shared symptom progression. Overall, genomic and phylogenomic analyses were insufficient to differentiate the Moko variants based on symptom progression. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500402 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500402 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2022-0038 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.45 n.4 2022 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122390713729024 |