Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015 |
Resumo: | Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes has been used as a powerful tool for functional studies. Most available information on gene function is based on heterologous expression systems. However, as the activity of many promoters or proteins frequently depends on specific interactions that only occur in homologous backgrounds, a final confirmation based on a homologous expression system is desirable. With respect to soybean biotech improvement, transgenic lines with agronomical, nutritional and pharmaceutical traits have been obtained, including herbicide-tolerant soybeans, which represented the principal biotech crop in 2011, occupying 47% of the global biotech area. |
id |
SBG-1_e3d2f2d0671e47b8cb98729f82a5c035 |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572012000600015 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plantstransgenic plantsparticle bombardmentAgrobacterium systemsfunctional analysisgenetic improvementTransgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes has been used as a powerful tool for functional studies. Most available information on gene function is based on heterologous expression systems. However, as the activity of many promoters or proteins frequently depends on specific interactions that only occur in homologous backgrounds, a final confirmation based on a homologous expression system is desirable. With respect to soybean biotech improvement, transgenic lines with agronomical, nutritional and pharmaceutical traits have been obtained, including herbicide-tolerant soybeans, which represented the principal biotech crop in 2011, occupying 47% of the global biotech area.Sociedade Brasileira de Genética2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015Genetics and Molecular Biology v.35 n.4 suppl.1 2012reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572012000600015info:eu-repo/semantics/openAccessHomrich,Milena SchenkelWiebke-Strohm,BeatrizWeber,Ricardo Luís MayerBodanese-Zanettini,Maria Helenaeng2012-12-18T00:00:00Zoai:scielo:S1415-47572012000600015Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2012-12-18T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants |
title |
Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants |
spellingShingle |
Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants Homrich,Milena Schenkel transgenic plants particle bombardment Agrobacterium systems functional analysis genetic improvement |
title_short |
Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants |
title_full |
Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants |
title_fullStr |
Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants |
title_full_unstemmed |
Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants |
title_sort |
Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants |
author |
Homrich,Milena Schenkel |
author_facet |
Homrich,Milena Schenkel Wiebke-Strohm,Beatriz Weber,Ricardo Luís Mayer Bodanese-Zanettini,Maria Helena |
author_role |
author |
author2 |
Wiebke-Strohm,Beatriz Weber,Ricardo Luís Mayer Bodanese-Zanettini,Maria Helena |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Homrich,Milena Schenkel Wiebke-Strohm,Beatriz Weber,Ricardo Luís Mayer Bodanese-Zanettini,Maria Helena |
dc.subject.por.fl_str_mv |
transgenic plants particle bombardment Agrobacterium systems functional analysis genetic improvement |
topic |
transgenic plants particle bombardment Agrobacterium systems functional analysis genetic improvement |
description |
Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes has been used as a powerful tool for functional studies. Most available information on gene function is based on heterologous expression systems. However, as the activity of many promoters or proteins frequently depends on specific interactions that only occur in homologous backgrounds, a final confirmation based on a homologous expression system is desirable. With respect to soybean biotech improvement, transgenic lines with agronomical, nutritional and pharmaceutical traits have been obtained, including herbicide-tolerant soybeans, which represented the principal biotech crop in 2011, occupying 47% of the global biotech area. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572012000600015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.35 n.4 suppl.1 2012 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122384961241088 |