Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500016 |
Resumo: | Previous work on gene expression analysis based on RNA sequencing identified a variety of differentially expressed cDNA fragments in the genic male sterile-fertile line 114AB of Capsicum annuum L. In this work, we examined the accumulation of one of the transcript-derived fragments (TDFs), CaMF3 (male fertile 3), in the flower buds of a fertile line. The full genomic DNA sequence of CaMF3 was 1,951 bp long and contained 6 exons and 5 introns, with the complete sequence encoding a putative 25.89 kDa protein of 234 amino acids. The predicted protein of CaMF3 shared sequence similarity with members of the isoamyl acetate-hydrolyzing esterase (IAH1) protein family. CaMF3 expression was detected only in flower buds at stages 7 and 8 and in open flowers of a male fertile line; no expression was observed in any organs of a male sterile line. Fine expression analysis revealed that CaMF3 was expressed specifically in anthers of the fertile line. These results suggest that CaMF3 is an anther-specific gene that may be essential for anther or pollen development in C. annuum. |
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Genetics and Molecular Biology |
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Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L.anther-specificCaMF3Capsicum annuum L. genic male sterilityIAH1Previous work on gene expression analysis based on RNA sequencing identified a variety of differentially expressed cDNA fragments in the genic male sterile-fertile line 114AB of Capsicum annuum L. In this work, we examined the accumulation of one of the transcript-derived fragments (TDFs), CaMF3 (male fertile 3), in the flower buds of a fertile line. The full genomic DNA sequence of CaMF3 was 1,951 bp long and contained 6 exons and 5 introns, with the complete sequence encoding a putative 25.89 kDa protein of 234 amino acids. The predicted protein of CaMF3 shared sequence similarity with members of the isoamyl acetate-hydrolyzing esterase (IAH1) protein family. CaMF3 expression was detected only in flower buds at stages 7 and 8 and in open flowers of a male fertile line; no expression was observed in any organs of a male sterile line. Fine expression analysis revealed that CaMF3 was expressed specifically in anthers of the fertile line. These results suggest that CaMF3 is an anther-specific gene that may be essential for anther or pollen development in C. annuum.Sociedade Brasileira de Genética2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500016Genetics and Molecular Biology v.35 n.4 2012reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572012005000057info:eu-repo/semantics/openAccessHao,XuefengChen,ChangmingChen,GuojuCao,BihaoChen,QinghuaLei,Jianjuneng2012-12-12T00:00:00Zoai:scielo:S1415-47572012000500016Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2012-12-12T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L. |
title |
Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L. |
spellingShingle |
Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L. Hao,Xuefeng anther-specific CaMF3 Capsicum annuum L. genic male sterility IAH1 |
title_short |
Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L. |
title_full |
Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L. |
title_fullStr |
Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L. |
title_full_unstemmed |
Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L. |
title_sort |
Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L. |
author |
Hao,Xuefeng |
author_facet |
Hao,Xuefeng Chen,Changming Chen,Guoju Cao,Bihao Chen,Qinghua Lei,Jianjun |
author_role |
author |
author2 |
Chen,Changming Chen,Guoju Cao,Bihao Chen,Qinghua Lei,Jianjun |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Hao,Xuefeng Chen,Changming Chen,Guoju Cao,Bihao Chen,Qinghua Lei,Jianjun |
dc.subject.por.fl_str_mv |
anther-specific CaMF3 Capsicum annuum L. genic male sterility IAH1 |
topic |
anther-specific CaMF3 Capsicum annuum L. genic male sterility IAH1 |
description |
Previous work on gene expression analysis based on RNA sequencing identified a variety of differentially expressed cDNA fragments in the genic male sterile-fertile line 114AB of Capsicum annuum L. In this work, we examined the accumulation of one of the transcript-derived fragments (TDFs), CaMF3 (male fertile 3), in the flower buds of a fertile line. The full genomic DNA sequence of CaMF3 was 1,951 bp long and contained 6 exons and 5 introns, with the complete sequence encoding a putative 25.89 kDa protein of 234 amino acids. The predicted protein of CaMF3 shared sequence similarity with members of the isoamyl acetate-hydrolyzing esterase (IAH1) protein family. CaMF3 expression was detected only in flower buds at stages 7 and 8 and in open flowers of a male fertile line; no expression was observed in any organs of a male sterile line. Fine expression analysis revealed that CaMF3 was expressed specifically in anthers of the fertile line. These results suggest that CaMF3 is an anther-specific gene that may be essential for anther or pollen development in C. annuum. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572012005000057 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.35 n.4 2012 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122385075535872 |