Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400490 |
Resumo: | Abstract Curcumin (CMN) is the principal active component derived from the rhizome of Curcuma longa (Curcuma longa L.). It is a liposoluble polyphenolic compound that possesses great therapeutic potential. Its clinical application is, however, limited by the low concentrations detected following oral administration. One key strategy for improving the solubility and bioavailability of poorly water-soluble drugs is solid dispersion, though it is not known whether this technique might influence the pharmacological effects of CMN. Thus, in this study, we aimed to evaluate the antioxidant and antigenotoxic effects of CMN formulated in a solid dispersion (CMN SD) compared to unmodified CMN delivered to Wistar rats. Cisplatin (cDDP) was used as the damage-inducing agent in these evaluations. The comet assay results showed that CMN SD was not able to reduce the formation of cDDP-DNA crosslinks, but it decreased the formation of micronuclei induced by cDDP and attenuated cDDP-induced oxidative stress. Furthermore, at a dose of 50 mg/kg b.w. both CMN SD and unmodified CMN increased the expression of Tp53 mRNA. Our results showed that CMN SD did not alter the antigenotoxic effects observed for unmodified CMN and showed effects similar to those of unmodified CMN for all of the parameters evaluated. In conclusion, CMN SD maintained the protective effects of unmodified CMN with the advantage of being chemically water soluble, with maximization of absorption in the gastrointestinal tract. Thus, the optimization of the physical and chemical properties of CMN SD may increase the potential for the therapeutic use of curcumin. |
id |
SBG-1_f307a68b27268edf32fc79c31846a41c |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572015000400490 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effectsCurcuma longaantigenotoxicitymicronucleus testDNA damagecomet assayAbstract Curcumin (CMN) is the principal active component derived from the rhizome of Curcuma longa (Curcuma longa L.). It is a liposoluble polyphenolic compound that possesses great therapeutic potential. Its clinical application is, however, limited by the low concentrations detected following oral administration. One key strategy for improving the solubility and bioavailability of poorly water-soluble drugs is solid dispersion, though it is not known whether this technique might influence the pharmacological effects of CMN. Thus, in this study, we aimed to evaluate the antioxidant and antigenotoxic effects of CMN formulated in a solid dispersion (CMN SD) compared to unmodified CMN delivered to Wistar rats. Cisplatin (cDDP) was used as the damage-inducing agent in these evaluations. The comet assay results showed that CMN SD was not able to reduce the formation of cDDP-DNA crosslinks, but it decreased the formation of micronuclei induced by cDDP and attenuated cDDP-induced oxidative stress. Furthermore, at a dose of 50 mg/kg b.w. both CMN SD and unmodified CMN increased the expression of Tp53 mRNA. Our results showed that CMN SD did not alter the antigenotoxic effects observed for unmodified CMN and showed effects similar to those of unmodified CMN for all of the parameters evaluated. In conclusion, CMN SD maintained the protective effects of unmodified CMN with the advantage of being chemically water soluble, with maximization of absorption in the gastrointestinal tract. Thus, the optimization of the physical and chemical properties of CMN SD may increase the potential for the therapeutic use of curcumin.Sociedade Brasileira de Genética2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400490Genetics and Molecular Biology v.38 n.4 2015reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-475738420150046info:eu-repo/semantics/openAccessMendonça,Leonardo MeneghinMachado,Carla da SilvaTeixeira,Cristiane Cardoso CorreiaFreitas,Luis Alexandre Pedro deBianchi,Maria Lourdes PiresAntunes,Lusânia Maria Greggieng2016-02-02T00:00:00Zoai:scielo:S1415-47572015000400490Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2016-02-02T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects |
title |
Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects |
spellingShingle |
Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects Mendonça,Leonardo Meneghin Curcuma longa antigenotoxicity micronucleus test DNA damage comet assay |
title_short |
Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects |
title_full |
Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects |
title_fullStr |
Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects |
title_full_unstemmed |
Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects |
title_sort |
Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects |
author |
Mendonça,Leonardo Meneghin |
author_facet |
Mendonça,Leonardo Meneghin Machado,Carla da Silva Teixeira,Cristiane Cardoso Correia Freitas,Luis Alexandre Pedro de Bianchi,Maria Lourdes Pires Antunes,Lusânia Maria Greggi |
author_role |
author |
author2 |
Machado,Carla da Silva Teixeira,Cristiane Cardoso Correia Freitas,Luis Alexandre Pedro de Bianchi,Maria Lourdes Pires Antunes,Lusânia Maria Greggi |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Mendonça,Leonardo Meneghin Machado,Carla da Silva Teixeira,Cristiane Cardoso Correia Freitas,Luis Alexandre Pedro de Bianchi,Maria Lourdes Pires Antunes,Lusânia Maria Greggi |
dc.subject.por.fl_str_mv |
Curcuma longa antigenotoxicity micronucleus test DNA damage comet assay |
topic |
Curcuma longa antigenotoxicity micronucleus test DNA damage comet assay |
description |
Abstract Curcumin (CMN) is the principal active component derived from the rhizome of Curcuma longa (Curcuma longa L.). It is a liposoluble polyphenolic compound that possesses great therapeutic potential. Its clinical application is, however, limited by the low concentrations detected following oral administration. One key strategy for improving the solubility and bioavailability of poorly water-soluble drugs is solid dispersion, though it is not known whether this technique might influence the pharmacological effects of CMN. Thus, in this study, we aimed to evaluate the antioxidant and antigenotoxic effects of CMN formulated in a solid dispersion (CMN SD) compared to unmodified CMN delivered to Wistar rats. Cisplatin (cDDP) was used as the damage-inducing agent in these evaluations. The comet assay results showed that CMN SD was not able to reduce the formation of cDDP-DNA crosslinks, but it decreased the formation of micronuclei induced by cDDP and attenuated cDDP-induced oxidative stress. Furthermore, at a dose of 50 mg/kg b.w. both CMN SD and unmodified CMN increased the expression of Tp53 mRNA. Our results showed that CMN SD did not alter the antigenotoxic effects observed for unmodified CMN and showed effects similar to those of unmodified CMN for all of the parameters evaluated. In conclusion, CMN SD maintained the protective effects of unmodified CMN with the advantage of being chemically water soluble, with maximization of absorption in the gastrointestinal tract. Thus, the optimization of the physical and chemical properties of CMN SD may increase the potential for the therapeutic use of curcumin. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400490 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400490 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-475738420150046 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.38 n.4 2015 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122386638962688 |