Protein profile of rice (Oryza sativa) seeds

Detalhes bibliográficos
Autor(a) principal: Yang,Yanhua
Data de Publicação: 2013
Outros Autores: Dai,Li, Xia,Hengchuan, Zhu,Keming, Liu,Haijun, Chen,Keping
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012
Resumo: Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic and were related to binding, catalytic, cellular or metabolic processes. These results expand our knowledge of the rice proteome and improve our understanding of the cellular biology of rice seeds.
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spelling Protein profile of rice (Oryza sativa) seedsmass spectrometryproteomic analysisrice seedtwo-dimensional electrophoresisSeeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic and were related to binding, catalytic, cellular or metabolic processes. These results expand our knowledge of the rice proteome and improve our understanding of the cellular biology of rice seeds.Sociedade Brasileira de Genética2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012Genetics and Molecular Biology v.36 n.1 2013reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572013000100012info:eu-repo/semantics/openAccessYang,YanhuaDai,LiXia,HengchuanZhu,KemingLiu,HaijunChen,Kepingeng2013-02-28T00:00:00Zoai:scielo:S1415-47572013000100012Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2013-02-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Protein profile of rice (Oryza sativa) seeds
title Protein profile of rice (Oryza sativa) seeds
spellingShingle Protein profile of rice (Oryza sativa) seeds
Yang,Yanhua
mass spectrometry
proteomic analysis
rice seed
two-dimensional electrophoresis
title_short Protein profile of rice (Oryza sativa) seeds
title_full Protein profile of rice (Oryza sativa) seeds
title_fullStr Protein profile of rice (Oryza sativa) seeds
title_full_unstemmed Protein profile of rice (Oryza sativa) seeds
title_sort Protein profile of rice (Oryza sativa) seeds
author Yang,Yanhua
author_facet Yang,Yanhua
Dai,Li
Xia,Hengchuan
Zhu,Keming
Liu,Haijun
Chen,Keping
author_role author
author2 Dai,Li
Xia,Hengchuan
Zhu,Keming
Liu,Haijun
Chen,Keping
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Yang,Yanhua
Dai,Li
Xia,Hengchuan
Zhu,Keming
Liu,Haijun
Chen,Keping
dc.subject.por.fl_str_mv mass spectrometry
proteomic analysis
rice seed
two-dimensional electrophoresis
topic mass spectrometry
proteomic analysis
rice seed
two-dimensional electrophoresis
description Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic and were related to binding, catalytic, cellular or metabolic processes. These results expand our knowledge of the rice proteome and improve our understanding of the cellular biology of rice seeds.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572013000100012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.36 n.1 2013
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
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institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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